多重 PCR 检测法鉴定三种主要致病性弧菌，即霍乱弧菌、副溶血性弧菌和创伤弧菌。
Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.
机构信息
Department of Bacteriology I, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan.
出版信息
Mol Cell Probes. 2011 Aug;25(4):174-6. doi: 10.1016/j.mcp.2011.04.004. Epub 2011 Apr 22.
PMID:21530641
Abstract
A multiplex PCR assay was developed based on atpA-sequence diversification for molecular identification of 3 major pathogenic Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. It specifically identified them from among 133 strains of various Vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.
摘要
建立了一种基于 atpA 序列多样性的多重 PCR 检测方法，用于分子鉴定 3 种主要致病性弧菌：霍乱弧菌、副溶血性弧菌和创伤弧菌。该方法能够特异性地从 133 株不同的弧菌属和其他属菌株中鉴定出它们，并且适用于检测海水，表明其具有实用性。
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