Zhang Yi, Ryan James A, Di Cesare Paul E, Liu Judy, Walsh Christopher A, You Zongbing
Lawrence Ellison Center for Tissue Regeneration and Repair, Department of Orthopaedic Surgery, School of Medicine, University of California-Davis, 4635 Second Avenue, Room 2000, Sacramento, CA 95817, USA.
Biochem Biophys Res Commun. 2007 Nov 23;363(3):694-700. doi: 10.1016/j.bbrc.2007.09.030. Epub 2007 Sep 19.
Articular cartilage and cartilage in the embryonic cartilaginous anlagen and growth plates are both hyaline cartilages. In this study, we found that doublecortin (DCX) was expressed in articular chondrocytes but not in chondrocytes from the cartilaginous anlagen or growth plates. DCX was expressed by the cells in the chondrogenous layers but not intermediate layer of joint interzone. Furthermore, the synovium and cruciate ligaments were DCX-negative. DCX-positive chondrocytes were very rare in tissue engineered cartilage derived from in vitro pellet culture of rat chondrosarcoma, ATDC5, and C3H10T1/2 cells. However, the new hyaline cartilage formed in rabbit knee defect contained mostly DCX-positive chondrocytes. Our results demonstrate that DCX can be used as a marker to distinguish articular chondrocytes from other chondrocytes and to evaluate the quality of tissue engineered or regenerated cartilage in terms of their "articular" or "non-articular" nature.
关节软骨以及胚胎软骨原基和生长板中的软骨均为透明软骨。在本研究中，我们发现双皮质素（DCX）在关节软骨细胞中表达，但在软骨原基或生长板的软骨细胞中不表达。DCX由关节间区软骨形成层的细胞表达，而中间层细胞不表达。此外，滑膜和交叉韧带DCX呈阴性。在源自大鼠软骨肉瘤、ATDC5和C3H10T1/2细胞体外微团培养的组织工程软骨中，DCX阳性软骨细胞非常罕见。然而，兔膝关节缺损处形成的新透明软骨大多含有DCX阳性软骨细胞。我们的结果表明，DCX可作为一种标志物，用于区分关节软骨细胞与其他软骨细胞，并根据组织工程或再生软骨的“关节”或“非关节”性质来评估其质量。
