Sørbø Jan Gunnar, Moe Svein Erik, Holen Torgeir
Center for Molecular Biology and Neuroscience (CMBN), University of Oslo, Norway.
FEBS Lett. 2007 Oct 16;581(25):4884-90. doi: 10.1016/j.febslet.2007.09.018. Epub 2007 Sep 19.
Aquaporin-4 (AQP4) has been reported to be upregulated post-partum in pregnancy and in early lung development. Several technical challenges exist in measuring AQP4 protein levels, among them sensitivity to detergent solubilization, sample heating and gel composition. Here we have optimized quantification of AQP4 using immuno-blots. Using improved methodology we find no evidence for AQP4 upregulation post-partum or in the early lung development. However, in the nasal epithelium AQP4 is upregulated as early as in the brain. Furthermore, AQP4 is strongly expressed in the glomerulus, the synaptic unit of the olfactory bulb, suggesting a role for AQP4 in olfactory function.
据报道，水通道蛋白4（AQP4）在产后、孕期及肺早期发育过程中表达上调。在测量AQP4蛋白水平时存在若干技术挑战，其中包括对去污剂溶解、样品加热及凝胶成分的敏感性。在此，我们利用免疫印迹法优化了AQP4的定量分析。采用改进方法后，我们未发现产后或肺早期发育过程中AQP4上调的证据。然而，在鼻上皮中，AQP4早在脑内时就已上调。此外，AQP4在肾小球（嗅球的突触单元）中强烈表达，提示AQP4在嗅觉功能中发挥作用。
