Xu Jun, Tan Tianwei, Janson Jan-Christer
Department of Bioengineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
J Chromatogr A. 2007 Oct 26;1169(1-2):235-8. doi: 10.1016/j.chroma.2007.08.085. Epub 2007 Sep 12.
(-)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum mobile phase compositions regarding purity, recovery and separation time of 78/22 (v/v). Maximum practical sample loading was 100 mg GTP per run (corresponding to 4.2 mg/ml Superose). An EGCG purity of 99% with recoveries in the range 60-65% was achieved in one step directly from the crude GTP extract. Full column regeneration was obtained using solvents in the following order: 0.5 M NaOH, distilled water and 30% acetic acid.
通过在Superose 12 HR 10/30柱上进行吸附色谱，一步从绿茶多酚（GTP）粗提物中纯化出（-）-表没食子儿茶素没食子酸酯（EGCG）。所用流动相为乙腈和水的混合物，就纯度、回收率和分离时间而言，最佳流动相组成是78/22（v/v）。每次运行的最大实际样品上样量为100 mg GTP（相当于4.2 mg/ml Superose）。直接从GTP粗提物一步获得了纯度为99%且回收率在60 - 65%范围内的EGCG。使用以下顺序的溶剂可实现柱的完全再生：0.5 M NaOH、蒸馏水和30%乙酸。
