Jin Yinzhe, Jin Chun Hua, Row Kyung Ho
Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 235 Yonghyun-dong, Nam-ku, Incheon 402-751, Korea.
Biotechnol J. 2006 Feb;1(2):209-13. doi: 10.1002/biot.200500019.
Catechin compounds from Korean and Chinese green tea, and pu-erh, Indian black, Longjing, Tieguanyin, Bamboo, Jasmine, Oolong, Flower, Red teas, as potential anticancer and antioxidant components, were target material in this work. After extracting the green tea with water at 50 degrees C for 4 h, the extract was partitioned with water/chloroform, which was best suited to remove caffeine impurity from the extract. Further, the resulting extract was partitioned with water/ethyl acetate to deeply purify the five catechin compounds epigallocatechin, (+) catechin, epicatechin, epigallocatechin gallate and epicatechin gallate. The extracted samples were analyzed by reversed-phase high performance liquid chromatography. The mobile phase applied was the binary system of A (water/acetic acid, 100/0.1 vol%) and B (acetonitrile/acetic acid 100/0.1 vol%) from 90:10 to 70:30 (A:B vol%) in a linear gradient over 30 min time. The amount of catechin compounds extracted from Chinese green tea was 114.65% higher than from the Korean green tea. Comparing various tea sorts, the green teas contained more than 1.7 times of the five catechin compounds contained in other teas.
来自韩国绿茶、中国绿茶、普洱茶、印度红茶、龙井、铁观音、竹叶青、茉莉花茶、乌龙茶、花茶、红茶的儿茶素化合物，作为潜在的抗癌和抗氧化成分，是本研究的目标物质。在50℃用水提取绿茶4小时后，提取物用水/氯仿进行分配，这最适合从提取物中去除咖啡因杂质。此外，所得提取物再用水/乙酸乙酯进行分配，以深度纯化表没食子儿茶素、（+）儿茶素、表儿茶素、表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯这五种儿茶素化合物。提取的样品通过反相高效液相色谱进行分析。所应用的流动相是A（水/乙酸，100/0.1体积%）和B（乙腈/乙酸100/0.1体积%）的二元体系，在30分钟内从90:10到70:30（A:B体积%）呈线性梯度变化。从中国绿茶中提取的儿茶素化合物的量比从韩国绿茶中提取的高114.65%。比较各种茶叶品种，绿茶中所含的五种儿茶素化合物比其他茶叶中的多1.7倍以上。
