马铃薯Y病毒的HC-Pro蛋白可在植物体内与三种拟南芥20S蛋白酶体亚基相互作用。
HC-Pro protein of Potato virus Y can interact with three Arabidopsis 20S proteasome subunits in planta.
作者信息
Jin Yongsheng, Ma Dongyuan, Dong Jiangli, Jin Jingchen, Li Daofeng, Deng Changwang, Wang Tao
机构信息
State Key Laboratory of Agro-biotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China.
出版信息
J Virol. 2007 Dec;81(23):12881-8. doi: 10.1128/JVI.00913-07. Epub 2007 Sep 26.
Abstract
The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 140 of PAA, 1 to 80 of PBB, and 160 to 274 of PBE are necessary for binding PAA, PBB, and PBE to PVY HC-Pro, respectively. Deletion mutant analysis of PVY HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. The ability of HC-Pro to interact and interfere with the activity of the 20S proteasome may help explain the molecular basis of its multifunctional character.
摘要
多功能蛋白辅助成分蛋白酶（HC-Pro）被认为会干扰20S蛋白酶体的活性；然而，尚未确定这两种蛋白质的相互作用位点。在此，我们首先利用酵母双杂交系统和双分子荧光互补分析表明，马铃薯Y病毒（PVY）的HC-Pro蛋白可与三种拟南芥20S蛋白酶体亚基（PAA、PBB和PBE）相互作用。此外，对20S蛋白酶体的几个突变亚基与PVY HC-Pro之间相互作用的酵母双杂交分析证实，PAA的81至140位残基、PBB的1至80位残基以及PBE的160至274位残基分别是PAA、PBB和PBE与PVY HC-Pro结合所必需的。PVY HC-Pro的缺失突变分析表明，其N端（1至97位残基）对于其与三种拟南芥20S蛋白酶体亚基的相互作用是必需的。HC-Pro与20S蛋白酶体相互作用并干扰其活性的能力可能有助于解释其多功能特性的分子基础。
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