在使用线性阵列HPV基因分型检测对肛门生殖器标本进行HPV感染筛查时，用于确认52型人乳头瘤病毒（HPV）感染的实时PCR检测法
Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test.
作者信息
Coutlée François, Rouleau Danielle, Ghattas Georges, Hankins Catherine, Vézina Sylvie, Coté Pierre, Macleod John, de Pokomandy Alexandra, Money Deborah, Walmsley Sharon, Voyer Hélène, Brassard Paul, Franco Eduardo
机构信息
Département de Microbiologie et Infectiologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.
出版信息
J Clin Microbiol. 2007 Nov;45(11):3821-3. doi: 10.1128/JCM.01145-07. Epub 2007 Sep 26.
Abstract
A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.
摘要
一种用于检测人乳头瘤病毒52型（HPV - 52）DNA的新型实时聚合酶链反应检测法（RT - 52）在265份肛门生殖器样本上进行了评估。与传统的HPV - 52分型检测法相比，RT - 52的灵敏度为98.4%，特异性为100%，传统检测法包括PGMY产物与HPV - 52特异性探针的杂交以及HPV - 52 E6的聚合酶链反应测序。
相似文献
1
Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test.在使用线性阵列HPV基因分型检测对肛门生殖器标本进行HPV感染筛查时，用于确认52型人乳头瘤病毒（HPV）感染的实时PCR检测法
J Clin Microbiol. 2007 Nov;45(11):3821-3. doi: 10.1128/JCM.01145-07. Epub 2007 Sep 26.
2
Universal ProbeLibrary based real-time PCR assay for detection and confirmation of human papillomavirus genotype 52 infections.基于通用探针库的实时 PCR 检测法用于检测和确认人乳头瘤病毒 52 型感染。
J Virol Methods. 2010 Feb;163(2):492-4. doi: 10.1016/j.jviromet.2009.10.024. Epub 2009 Oct 31.
3
Analytical evaluation of the PapilloCheck test, a new commercial DNA chip for detection and genotyping of human papillomavirus.对PapilloCheck检测法的分析评估，一种用于检测人乳头瘤病毒并进行基因分型的新型商用DNA芯片。
J Virol Methods. 2009 Mar;156(1-2):77-83. doi: 10.1016/j.jviromet.2008.11.002. Epub 2008 Dec 17.
4
Detection of human papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to the hybrid capture II and MY-PCR assays and a two-step nested PCR assay.宫颈样本中人乳头瘤病毒DNA的检测：新型PGMY-PCR与杂交捕获II和MY-PCR检测以及两步巢式PCR检测的比较分析
J Clin Microbiol. 2004 Aug;42(8):3861-4. doi: 10.1128/JCM.42.8.3861-3864.2004.
5
Detection and genotyping of human papillomavirus by real-time PCR assay.实时聚合酶链反应检测和人乳头瘤病毒基因分型。
J Clin Virol. 2013 Mar;56(3):244-9. doi: 10.1016/j.jcv.2012.11.003. Epub 2012 Nov 22.
6
Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the Linear array HPV genotyping test.采用PGMY引物和线性阵列HPV基因分型检测法增强对肛门生殖器样本中人乳头瘤病毒（HPV）DNA的检测及分型
J Clin Microbiol. 2006 Jun;44(6):1998-2006. doi: 10.1128/JCM.00104-06.
7
Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR.基于聚合酶链反应的基因分型阵列进行人乳头瘤病毒分型并与型特异性聚合酶链反应相比较。
J Clin Virol. 2008 Aug;42(4):361-7. doi: 10.1016/j.jcv.2008.03.018. Epub 2008 May 2.
8
Human papillomavirus oncogene mRNA testing for the detection of anal dysplasia in HIV-positive men who have sex with men.人乳头瘤病毒致癌基因 mRNA 检测在 HIV 阳性男男性行为者肛门发育不良中的应用。
J Clin Virol. 2012 Apr;53(4):325-31. doi: 10.1016/j.jcv.2011.12.029. Epub 2012 Jan 18.
9
Detection and identification of human papillomavirus using a PCR-restriction fragment mass polymorphism assay.采用 PCR-限制性片段质量多态性分析检测和鉴定人乳头瘤病毒。
Mol Med Rep. 2011 Jul-Aug;4(4):645-50. doi: 10.3892/mmr.2011.475. Epub 2011 Apr 14.
10
Nonisotopic detection of human papillomavirus DNA in clinical specimens using a consensus PCR and a generic probe mix in an enzyme-linked immunosorbent assay format.在酶联免疫吸附测定形式下，使用共识聚合酶链反应和通用探针混合物对临床标本中的人乳头瘤病毒DNA进行非同位素检测。
J Clin Microbiol. 2001 Oct;39(10):3530-6. doi: 10.1128/JCM.39.10.3530-3536.2001.
引用本文的文献
1
Protection Against Persistent HPV-16/18 Infection After Different Number of Doses of Quadrivalent Vaccine in Girls and Young Women: A Randomized Clinical Trial.不同剂量四价疫苗对女孩和年轻女性持续性人乳头瘤病毒16/18感染的预防作用：一项随机临床试验
JAMA Netw Open. 2025 Jul 1;8(7):e2519095. doi: 10.1001/jamanetworkopen.2025.19095.
2
Viral Load of Human Papillomavirus (HPV) During Pregnancy and Its Association With HPV Vertical Transmission.妊娠期人乳头瘤病毒（HPV）病毒载量及其与HPV垂直传播的关联
J Med Virol. 2025 Feb;97(2):e70221. doi: 10.1002/jmv.70221.
3
Comparative evaluation of HPV genotyping: A study on the performance concordance between Anyplex II HPV28 detection and Linear Array genotyping tests in nationwide studies in Brazil.HPV 基因分型的比较评估：巴西全国性研究中 Anyplex II HPV28 检测与线性阵列基因分型检测性能一致性研究。
PLoS One. 2024 Jun 11;19(6):e0305122. doi: 10.1371/journal.pone.0305122. eCollection 2024.
4
Identifying risk factors for prevalent anal human papillomavirus type 16 infection in women living with HIV.确定人乳头瘤病毒 16 型在 HIV 感染者女性中流行感染的风险因素。
PLoS One. 2022 May 19;17(5):e0268521. doi: 10.1371/journal.pone.0268521. eCollection 2022.
5
Human papillomavirus genotype concordance between Anyplex II HPV28 and linear array HPV genotyping test in anogenital samples.肛门生殖器样本中Anyplex II HPV28与线性阵列HPV基因分型检测之间的人乳头瘤病毒基因型一致性
J Med Virol. 2022 Jun;94(6):2824-2832. doi: 10.1002/jmv.27605. Epub 2022 Jan 28.
6
Human papillomavirus genotype distribution and factors associated among female sex workers in West Africa.西非女性性工作者中人类乳头瘤病毒基因型分布及相关因素。
PLoS One. 2020 Nov 25;15(11):e0242711. doi: 10.1371/journal.pone.0242711. eCollection 2020.
7
Antibodies to human papillomavirus types 6, 11, 16 and 18: Vertical transmission and clearance in children up to two years of age.人乳头瘤病毒6型、11型、16型和18型抗体：两岁以下儿童中的垂直传播与清除情况
EClinicalMedicine. 2020 Apr 7;21:100334. doi: 10.1016/j.eclinm.2020.100334. eCollection 2020 Apr.
8
Exploration of the effect of human papillomavirus (HPV) vaccination in a cohort of pregnant women in Montreal, 2010-2016.2010 - 2016年蒙特利尔孕妇队列中人乳头瘤病毒（HPV）疫苗接种效果探索
Heliyon. 2019 Aug 14;5(8):e02150. doi: 10.1016/j.heliyon.2019.e02150. eCollection 2019 Aug.
9
Human papillomavirus (HPV) perinatal transmission and risk of HPV persistence among children: Design, methods and preliminary results of the HERITAGE study.人乳头瘤病毒（HPV）围产期传播及儿童HPV持续感染风险：HERITAGE研究的设计、方法及初步结果
Papillomavirus Res. 2016 Dec;2:145-152. doi: 10.1016/j.pvr.2016.07.001. Epub 2016 Jul 12.
10
Low prevalence of vaccine-type HPV infections in young women following the implementation of a school-based and catch-up vaccination in Quebec, Canada.在加拿大魁北克省实施基于学校的和补充性疫苗接种后，年轻女性中疫苗型 HPV 感染的流行率较低。
Hum Vaccin Immunother. 2018 Jan 2;14(1):118-123. doi: 10.1080/21645515.2017.1385688. Epub 2017 Dec 13.
本文引用的文献
1
Clinical utility of HPV genotyping.人乳头瘤病毒基因分型的临床应用价值
Gynecol Oncol. 2006 Oct;103(1):12-7. doi: 10.1016/j.ygyno.2006.07.031. Epub 2006 Aug 24.
2
Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the Linear array HPV genotyping test.采用PGMY引物和线性阵列HPV基因分型检测法增强对肛门生殖器样本中人乳头瘤病毒（HPV）DNA的检测及分型
J Clin Microbiol. 2006 Jun;44(6):1998-2006. doi: 10.1128/JCM.00104-06.
3
Worldwide distribution of human papillomavirus types in cytologically normal women in the International Agency for Research on Cancer HPV prevalence surveys: a pooled analysis.国际癌症研究机构人乳头瘤病毒流行率调查中细胞学正常女性人乳头瘤病毒类型的全球分布：一项汇总分析
Lancet. 2005;366(9490):991-8. doi: 10.1016/S0140-6736(05)67069-9.
4
Sequence variation in primer targets affects the accuracy of viral quantitative PCR.引物靶标的序列变异会影响病毒定量PCR的准确性。
J Clin Virol. 2005 Oct;34(2):104-7. doi: 10.1016/j.jcv.2005.02.010.
5
The p53 codon 72 polymorphism and risk of high-grade cervical intraepithelial neoplasia.p53基因第72位密码子多态性与高级别宫颈上皮内瘤变风险
Cancer Detect Prev. 2005;29(4):307-16. doi: 10.1016/j.cdp.2005.06.007.
6
The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice.16或18型人乳头瘤病毒（HPV）感染女性发生宫颈上皮内瘤变和宫颈癌的10年风险升高以及型特异性HPV检测在临床实践中的潜在应用价值
J Natl Cancer Inst. 2005 Jul 20;97(14):1072-9. doi: 10.1093/jnci/dji187.
7
Human papillomavirus type 16 infections and 2-year absolute risk of cervical precancer in women with equivocal or mild cytologic abnormalities.16型人乳头瘤病毒感染与细胞学异常不明确或轻度异常女性患宫颈上皮内瘤变的2年绝对风险
J Natl Cancer Inst. 2005 Jul 20;97(14):1066-71. doi: 10.1093/jnci/dji186.
8
Self-sampling is associated with increased detection of human papillomavirus DNA in the genital tract of HIV-seropositive women.自我采样与提高HIV血清阳性女性生殖道中人乳头瘤病毒DNA的检测率相关。
Clin Infect Dis. 2005 Aug 15;41(4):527-34. doi: 10.1086/432059. Epub 2005 Jul 14.
9
Human papillomavirus genotype distribution in low-grade cervical lesions: comparison by geographic region and with cervical cancer.低级别宫颈病变中人类乳头瘤病毒基因型分布：按地理区域及与宫颈癌的比较
Cancer Epidemiol Biomarkers Prev. 2005 May;14(5):1157-64. doi: 10.1158/1055-9965.EPI-04-0812.
10
Epidemiologic profile of type-specific human papillomavirus infection and cervical neoplasia in Guanacaste, Costa Rica.哥斯达黎加瓜纳卡斯特省特定类型人乳头瘤病毒感染与宫颈肿瘤的流行病学概况
J Infect Dis. 2005 Jun 1;191(11):1796-807. doi: 10.1086/428850. Epub 2005 May 2.
Zotero 插件