Trottier Helen, Mayrand Marie-Hélène, Coutlée François, Monnier Patricia, Laporte Louise, Niyibizi Joseph, Carceller Ana-Maria, Fraser William D, Brassard Paul, Lacroix Jacques, Francoeur Diane, Bédard Marie-Josée, Girard Isabelle, Audibert François
Department of Social and Preventive Medicine, Université de Montréal, Sainte-Justine Hospital, Montreal, Canada.
Departments of Obstetrics and Gynecology and Department of Social and Preventive Medicine, Université de Montréal and CRCHUM, Montreal, Canada.
Papillomavirus Res. 2016 Dec;2:145-152. doi: 10.1016/j.pvr.2016.07.001. Epub 2016 Jul 12.
Perinatal route of transmission of human papillomavirus (HPV) has been demonstrated in several small studies. We designed a large prospective cohort study (HERITAGE) to better understand perinatal HPV. The objective of this article is to present the study design and preliminary data. In the first phase of the study, we recruited 167 women in Montreal, Canada, during the first trimester of pregnancy. An additional 850 are currently being recruited in the ongoing phase. Cervicovaginal samples were obtained from mothers in the first trimester and tested for HPV DNA from 36 mucosal genotypes (and repeated in the third trimester for HPV-positive mothers). Placental samples were also taken for HPV DNA testing. Conjunctival, oral, pharyngeal and genital samples were collected for HPV DNA testing in children of HPV-positive mothers at every 3-6 months from birth until 2 years of age. Blood samples were collected in mother and children for HPV serology testing. We found a high prevalence of HPV in pregnant women (45%[95%CI:37-53%]) and in placentas (14%[8-21%]). The proportion of HPV positivity (any site) among children at birth/3-months was 11%[5-22%]. HPV was detected in children in multiple sites including the conjunctiva (5%[10-14%]). The ongoing HERITAGE cohort will help provide a better understanding of perinatal HPV.
人乳头瘤病毒（HPV）围产期传播途径已在多项小型研究中得到证实。我们设计了一项大型前瞻性队列研究（HERITAGE），以更好地了解围产期HPV。本文的目的是介绍研究设计和初步数据。在研究的第一阶段，我们在加拿大蒙特利尔招募了167名怀孕头三个月的女性。在进行中的阶段，目前正在额外招募850名女性。在孕早期从母亲处获取宫颈阴道样本，并检测36种黏膜基因型的HPV DNA（HPV阳性母亲在孕晚期重复检测）。还采集胎盘样本进行HPV DNA检测。在HPV阳性母亲的孩子出生至2岁期间，每3至6个月收集结膜、口腔、咽部和生殖器样本进行HPV DNA检测。采集母亲和孩子的血液样本进行HPV血清学检测。我们发现孕妇（45%[95%CI：37 - 53%]）和胎盘（14%[8 - 21%]）中HPV的患病率很高。出生/3个月时儿童中HPV阳性（任何部位）的比例为11%[5 - 22%]。在包括结膜（5%[10 - 14%]）在内的多个部位检测到儿童感染HPV。正在进行的HERITAGE队列研究将有助于更好地了解围产期HPV。
