Komeda Y, Suzuki H, Ishidsu J I, Iino T
Mol Gen Genet. 1976 Dec 31;142(4):289-98. doi: 10.1007/BF00271253.
A mutational alteration either in adenylate cyclase (cya-) or in cyclic-3'5'-AMP (cAMP) receptor protein (crp-) rendered Salmonella typhimurium incapable of producing flagella. The amount of mRNA specific for flagellin in these mutants was almost negligible when assayed in an in vitro protein synthesizing system. A secondary mutation cfs, partially suppressing the cya- mutation, was identified among the revertants of cya-. A mutation in the same cistron as cfs resulted in a non-flagellate phenotype either by itself or in combination with cfs. The cistron, which was given the gene symbol flaT, was located between flaE and flaL. It was suggested that cAMP receptor protein together with cAMP modulates the gene flaT, which in turn acts as a positive effector on the synthesis of active mRNA specific for flagellin.
腺苷酸环化酶（cya-）或环-3',5'-AMP（cAMP）受体蛋白（crp-）的突变改变使鼠伤寒沙门氏菌无法产生鞭毛。当在体外蛋白质合成系统中进行检测时，这些突变体中鞭毛蛋白特异性mRNA的量几乎可以忽略不计。在cya-的回复突变体中鉴定出了一个部分抑制cya-突变的二次突变cfs。与cfs位于同一顺反子中的突变单独或与cfs组合都会导致无鞭毛表型。该顺反子被赋予基因符号flaT，位于flaE和flaL之间。有人提出，cAMP受体蛋白与cAMP一起调节flaT基因，而flaT基因又对鞭毛蛋白特异性活性mRNA的合成起正效应作用。
