Kustimur Semra, Kalkanci Ayse, Akbulut Gonca, Gonul Bilge, Bulduk Emre, Aksakal F Nur, Yetkin Ilhan
Department of Microbiology, Faculty of Medicine, Gazi University, Besevler, Ankara 06500, Turkey.
Mycopathologia. 2007 Nov;164(5):217-24. doi: 10.1007/s11046-007-9058-z.
The aim of this study is to determine the relation between diabetes and vaginal candidiasis in terms of oxidative biomarker levels in a vaginal candidiasis model of the diabetic rats by evaluating malondialdehyde (MDA), sulphydrile groups or glutathione (RSH), and ascorbic acid (C vit) levels. All rats were randomly divided into five groups. All of the groups were observed for 21 days. In the treated diabetes groups, MDA (0.90, 0.68 nmol/ml and 3.78, 3.79 nmol/g tissue, plasma and vaginal tissue, respectively) and RSH (227, 171 nmol/100 ml 0.38, 0.37 micromol/g tissue, plasma and vaginal tissue, respectively) levels were found to be decreased while the levels of C vit were found to be increased (0.49, 0.37 micromol/l 2.39, 2.01 nmol/g tissue plasma, and vaginal tissue, respectively) (P < 0.05). In the groups of untreated diabetes, vaginal candidiasis were found to be more serious and oxidative biomarkers were found to be increased (MDA 1.30, 1.26 nmol/ml and 7.82, 2.37 nmol/g tissue and RSH 258, 145 nmol/100 ml and 0.31, 0.46 micromol/g tissue) while the antioxidant C vit levels were found to be decreased (0.24, 0.17 micromol/l 1.33, 2.66 nmol/g tissue) (P < 0.05). RSH, plasma MDA, blood glucose, and tissue MDA levels of vaginal candidiasis embedeled diabetic rats, were found to be higher than those in untreated diabetic and untreated vaginitis enbedeled rats 'P < 0.05'. Vaginal candidiasis caused oxidative stress in diabetic rats working together. Systemic oxidative stress biomarkers were found to be affected from vaginal candidiasis although it was a local mucosal infection.
本研究的目的是通过评估丙二醛（MDA）、巯基或谷胱甘肽（RSH）以及抗坏血酸（维生素C）水平，在糖尿病大鼠的阴道念珠菌病模型中确定糖尿病与阴道念珠菌病之间在氧化生物标志物水平方面的关系。所有大鼠随机分为五组。所有组均观察21天。在治疗的糖尿病组中，发现MDA（分别为0.90、0.68 nmol/ml和3.78、3.79 nmol/g组织、血浆和阴道组织）和RSH（分别为227、171 nmol/100 ml和0.38、0.37 μmol/g组织、血浆和阴道组织）水平降低，而维生素C水平升高（分别为0.49、0.37 μmol/l和2.39、2.01 nmol/g组织、血浆和阴道组织）（P<0.05）。在未治疗的糖尿病组中，发现阴道念珠菌病更严重，氧化生物标志物升高（MDA为1.30、1.26 nmol/ml和7.82、2.37 nmol/g组织，RSH为258、145 nmol/100 ml和0.31、0.46 μmol/g组织），而抗氧化剂维生素C水平降低（0.24、0.17 μmol/l和1.33、2.66 nmol/g组织）（P<0.05）。阴道念珠菌病合并糖尿病大鼠的RSH、血浆MDA、血糖和组织MDA水平高于未治疗的糖尿病大鼠和未治疗的阴道炎大鼠（P<0.05）。阴道念珠菌病在糖尿病大鼠中共同引起氧化应激。尽管阴道念珠菌病是局部黏膜感染，但发现全身氧化应激生物标志物受到其影响。
