Advanced Materials Research Chair, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Mar 15;105:412-7. doi: 10.1016/j.saa.2012.11.078. Epub 2012 Dec 5.
A flow injection (FI) method is reported for the determination of folic acid by chemiluminescence method. This method is based on the reaction of folic acid with Ru(bipy)(3)(2+) and Ce(IV) to produce chemiluminescence. The calibration curve was linear over the range of 2.5×10(-5)-3.1×10(-7) mol/L with a detection limit of 2.3×10(-8) mol/L (S/N=3). The relative standard deviation of 1.0×10(-6) mol/L folic acid was found 3.5% (n=11). The influences of potential interfering substances were studied. The recovery was higher than 95.3%. The method was accurate, sensitive, and effective for assay of folic acid. This CL method was successfully applied to the determination of folic acid in pharmaceutical preparations. The mechanism of CL reaction was also studied.
一种流动注射（FI）方法被报道用于通过化学发光法测定叶酸。该方法基于叶酸与 Ru(bipy)(3)(2+)和 Ce(IV)反应产生化学发光。校准曲线在 2.5×10(-5)-3.1×10(-7) mol/L 范围内呈线性，检测限为 2.3×10(-8) mol/L（S/N=3）。在 1.0×10(-6) mol/L 叶酸的相对标准偏差为 3.5%（n=11）。研究了潜在干扰物质的影响。回收率高于 95.3%。该方法准确、灵敏、有效，可用于测定叶酸。该 CL 方法成功应用于药物制剂中叶酸的测定。还研究了 CL 反应的机制。
