Pell Andrew J, Keeler James
University of Cambridge, Department of Chemistry, Cambridge, UK.
J Magn Reson. 2007 Dec;189(2):293-9. doi: 10.1016/j.jmr.2007.09.002. Epub 2007 Sep 8.
Two-dimensional J-spectroscopy offers the possibility of a complete separation of chemical shifts and J-couplings. However, the usefulness of the experiment is considerably reduced by the fact that peaks in the spectra have the phase-twist lineshape. We present a simple new spectroscopic method for recording J-spectra in which the peaks are both in the absorption mode and retain their natural intensities, albeit at the cost of a considerable reduction in the signal-to-noise ratio. No special data-processing is required. The method is tested on quinine, and the steroid dehydroisoandrosterone.
二维J谱提供了将化学位移和J耦合完全分离的可能性。然而，由于谱图中的峰具有相位扭曲线形，该实验的实用性大大降低。我们提出了一种简单的新光谱方法来记录J谱，其中峰均处于吸收模式并保留其天然强度，尽管代价是信噪比大幅降低。无需特殊的数据处理。该方法在奎宁和甾体脱氢表雄酮上进行了测试。
