使用染料摄取试验测定印度单纯疱疹病毒株中的阿昔洛韦耐药性。

Aciclovir resistance among Indian strains of Herpes simplex virus as determined using a dye uptake assay.

作者信息

Abraham A M, Kavitha S, Joseph P, George R, Pillay D, Malathi J, Jesudason M V, Sridharan G

机构信息

Department of Clinical Virology, Christian Medical College, Vellore - 632 004, Tamil Nadu, India.

出版信息

Indian J Med Microbiol. 2007 Jul;25(3):260-2. doi: 10.4103/0255-0857.34771.

DOI:10.4103/0255-0857.34771

PMID:17901647

Abstract

Resistance to aciclovir (ACV) among Herpes simplex virus (HSV) isolates is increasingly being reported in the literature particularly in immunocompromised patients. However, there is only limited data available from India despite widespread use of ACV in our hospital. A cross-sectional study was hence conducted to determine the aciclovir (ACV) susceptibility of HSV 1 and 2 isolates using a dye uptake (DU) assay. This study showed a 3.0% prevalence of ACV resistance among HSV-1 strains (2/66, median IC 50 0.098 microg/mL) while in HSV-2 strains, it was 7.8% (5/64, median IC 50 0.195 microg/mL). The IC 50 for the HSV-1 and HSV-2 strains resistant to ACV was greater than or equal to 6.25 microg/mL.

摘要

文献中越来越多地报道了单纯疱疹病毒（HSV）分离株对阿昔洛韦（ACV）产生耐药性的情况，尤其是在免疫功能低下的患者中。然而，尽管我院广泛使用阿昔洛韦，但来自印度的相关数据却非常有限。因此，我们开展了一项横断面研究，采用染料摄取（DU）试验来确定HSV 1和2分离株对阿昔洛韦（ACV）的敏感性。该研究表明，HSV-1毒株中阿昔洛韦耐药率为3.0%（2/66，IC50中位数为0.098μg/mL），而在HSV-2毒株中，耐药率为7.8%（5/64，IC50中位数为0.195μg/mL）。对阿昔洛韦耐药的HSV-1和HSV-2毒株的IC50大于或等于6.25μg/mL。