临床标本和实验室分离株中阿昔洛韦耐药单纯疱疹病毒的频率
Frequency of acyclovir-resistant herpes simplex virus in clinical specimens and laboratory isolates.
作者信息
Shin Y K, Cai G Y, Weinberg A, Leary J J, Levin M J
机构信息
Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea.
出版信息
J Clin Microbiol. 2001 Mar;39(3):913-7. doi: 10.1128/JCM.39.3.913-917.2001.
The proportion of acyclovir (ACV)-resistant herpes simplex virus (HSV) isolates in clinical specimens and laboratory isolates was determined. HSV isolates in clinical specimens and laboratory isolates were cultured in the absence or presence of 5 microg of ACV per ml. The frequency of ACV-resistant HSV was calculated as (average virus titer in the wells with ACV)/(average virus titer in the wells without ACV). The mutation frequency of HSV type 1 isolates in clinical samples (directly from patient lesions) was 7.5 x 10(-4) +/- 2.5 x 10(-4) (mean +/- standard error), and that of HSV type 2 isolates was 15.0 x 10(-4) +/- 4.6 x 10(-4). The mutation frequencies of isolates derived in the laboratory from these clinical samples were very similar. Similarly, the 50% inhibitory concentrations for isolates in clinical samples and laboratory isolates were identical. The frequencies of ACV-resistant HSV types 1 and 2 were in a narrow range of 7.5 x 10(-4) to 15.0 x 10(-4) among isolates in clinical specimens and did not change for laboratory-derived pools of viral isolates.
测定了临床标本和实验室分离株中对阿昔洛韦（ACV）耐药的单纯疱疹病毒（HSV）分离株的比例。临床标本和实验室分离株中的HSV分离株在每毫升含5微克ACV或不含ACV的情况下进行培养。ACV耐药HSV的频率计算为（含ACV孔中的平均病毒滴度）/（不含ACV孔中的平均病毒滴度）。临床样本（直接取自患者病变）中1型HSV分离株的突变频率为7.5×10⁻⁴±2.5×10⁻⁴（平均值±标准误差），2型HSV分离株的突变频率为15.0×10⁻⁴±4.6×10⁻⁴。从这些临床样本在实验室中衍生的分离株的突变频率非常相似。同样，临床样本和实验室分离株中分离株的50%抑制浓度相同。临床标本中1型和2型ACV耐药HSV的频率在7.5×10⁻⁴至15.0×10⁻⁴的狭窄范围内，并且对于实验室衍生的病毒分离株库没有变化。
Zotero 插件