Dupouy J P
Neuroendocrinology. 1975;19(4):303-13. doi: 10.1159/000122451.
The corticosterone content of the adrenal glands was determined in 21-day-old rat fetuses: (1) before and after encephalectomy or decapitation (hypophysectomy); (2) after ACTH treatment of the encephalectomized or decapitated (hypophysectomized) fetuses; and (3) after administration of crude extracts (0.1 N HCl) of hypothalamic or cortical tissue from 20-day-old rat fetuses. A peak in the corticosterone content of the adrenals was observed 10 min after ACTH injection to enceaphlectomized or decapitated fetuses. A rise in corticosterone concentration was noted 5 and 10 min after the encephalectomized fetuses were given an injection of hypothalamic extract. This extract was devoid of appreciable ACTH activity when tested in decapitated fetuses. Cortical extract was inactive in encephalectomized or decapitated fetuses. These data suggest that fetal hypothalamic extract contains some CRF activity and that the fetal pituitary gland is responsive to the CRF.
测定了21日龄大鼠胎儿肾上腺中的皮质酮含量：(1) 在脑切除或断头（垂体切除）前后；(2) 对脑切除或断头（垂体切除）的胎儿进行促肾上腺皮质激素（ACTH）治疗后；以及(3) 给予20日龄大鼠胎儿下丘脑或皮质组织的粗提物（0.1N盐酸）后。在对脑切除或断头的胎儿注射ACTH后10分钟，观察到肾上腺皮质酮含量出现峰值。在给脑切除的胎儿注射下丘脑提取物后5分钟和10分钟，皮质酮浓度升高。当在断头胎儿中进行测试时，这种提取物没有明显的ACTH活性。皮质提取物对脑切除或断头的胎儿没有活性。这些数据表明，胎儿下丘脑提取物含有一些促肾上腺皮质激素释放因子（CRF）活性，并且胎儿垂体对CRF有反应。
