Watkins W B, Choy V J
Cell Tissue Res. 1979 Mar 19;197(2):337-46. doi: 10.1007/BF00233924.
Transverse sections of the median eminence from fetal and neonatal rats were examined by the immunoperoxidase technique to detect the presence of oxytocin, vasopressin and neurophysin. Neurophysin was observed in the 18-day fetus. Vasopressin and oxytocin were not detected until after birth, on the 4th and 8th days respectively. There was an accumulation of material crossreactive with neurophysin and vasopressin antibodies in the palisade layer of the median eminence between the 4th and 9th days after birth. This distribution of immunoreactive material in the palisade layer was suggestive of neurosecretory substances localized in two fibre tracts on either side of the median eminence. The data are consistent with the accumulation of corticotropin releasing factor and an associated neurophysin in this area. It is suggested that the accumulation of material occurs because of the relative immaturity of the capillary loops that constitute the primary plexus of the hypophysial portal system.
采用免疫过氧化物酶技术检查胎鼠和新生鼠正中隆起的横切片，以检测催产素、血管加压素和神经垂体素的存在。在18日龄胎儿中观察到了神经垂体素。血管加压素和催产素分别在出生后第4天和第8天才能检测到。出生后第4天至第9天，正中隆起的栅状层中存在与神经垂体素和血管加压素抗体发生交叉反应的物质积累。这种免疫反应性物质在栅状层的分布提示神经分泌物质定位于正中隆起两侧的两条纤维束中。这些数据与该区域促肾上腺皮质激素释放因子和相关神经垂体素的积累一致。有人认为，物质的积累是由于构成垂体门脉系统初级丛的毛细血管袢相对不成熟所致。
