Shah Zahoor Ahmad, Namiranian Khodadad, Klaus Judy, Kibler Kathy, Doré Sylvain
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
J Stroke Cerebrovasc Dis. 2006 Jul-Aug;15(4):133-8. doi: 10.1016/j.jstrokecerebrovasdis.2006.04.002.
Intraluminal occlusion of the middle cerebral artery in rodents is widely used for investigating cerebral ischemia and reperfusion injury. Two types of filaments used for occlusion were tested in terms of surgical success, incidence of subarachnoid hemorrhage, and mortality: a standard 6-0 monofilament coated with methyl methacrylate glue (rigid probe) and an 8-0 monofilament coated with silicone (flexible probe). In 98 wild-type (WT) mice, the flexible probe produced significantly (P < .05) more successful strokes (73.5%) than the rigid probe (46.6%). The incidences of subarachnoid hemorrhage (3.7%) and mortality (5.6%) with the flexible probe were significantly lower than those with the rigid probe (26.6% and 11.1%, respectively). Rigid and flexible probes were also compared in heme oxygenase 1 knockout (n = 17) and WT littermates (n = 17), because knockout mice have been suggested to have more fragile blood vessels. All mice receiving the flexible probe had successful strokes, with no cases of subarachnoid hemorrhage or mortality; however, with the rigid probe, the success rate was only 80% in the WT mice and 60% in the knockout mice. The rates of subarachnoid hemorrhage and mortality were also significantly higher with the rigid probe in both genotypes, but the infarct volumes produced by each type of probe did not differ significantly between the 2 groups. We conclude that the flexible silicone-coated 8-0 probe is superior to the more rigid glue-coated probe, because it produces infarct volumes of equal size with a higher success rate and lower risk of subarachnoid hemorrhage and mortality.
啮齿动物大脑中动脉腔内闭塞被广泛用于研究脑缺血和再灌注损伤。我们对用于闭塞的两种类型的细丝在手术成功率、蛛网膜下腔出血发生率和死亡率方面进行了测试：一种是涂有甲基丙烯酸甲酯胶水的标准6-0单丝（刚性探针），另一种是涂有硅酮的8-0单丝（柔性探针）。在98只野生型（WT）小鼠中，柔性探针产生的成功中风率（73.5%）显著高于刚性探针（46.6%）（P <.05）。柔性探针的蛛网膜下腔出血发生率（3.7%）和死亡率（5.6%）显著低于刚性探针（分别为26.6%和11.1%）。还对血红素加氧酶1基因敲除小鼠（n = 17）和野生型同窝小鼠（n = 17）使用刚性和柔性探针进行了比较，因为有人提出基因敲除小鼠的血管更脆弱。所有接受柔性探针的小鼠都成功发生了中风，没有蛛网膜下腔出血或死亡病例；然而，使用刚性探针时，野生型小鼠的成功率仅为80%，基因敲除小鼠为60%。两种基因型中，刚性探针的蛛网膜下腔出血率和死亡率也显著更高，但两种探针产生的梗死体积在两组之间没有显著差异。我们得出结论，涂有硅酮的柔性8-0探针优于刚性更强的涂胶探针，因为它能产生大小相同的梗死体积，成功率更高，蛛网膜下腔出血和死亡风险更低。
