Auriat Angela M, Colbourne Frederick
Department of Psychology, University of Alberta, Edmonton, Alta., Canada.
Behav Brain Res. 2008 Jan 25;186(2):222-9. doi: 10.1016/j.bbr.2007.08.010. Epub 2007 Aug 19.
D-amphetamine (AMP) paired with physical activity (e.g., beam walking) improves recovery after ischemic injury to the cortical motor system of rodents. We tested whether AMP promotes recovery after intracerebral hemorrhage (ICH) in rats. A moderate-sized ICH was produced by stereotaxically injecting collagenase into the striatum. Five days later rats were placed into either environmental enrichment cages (EE) or a control condition (group housing in standard cages) until euthanasia at 4 weeks post-ICH. Animals were injected with either AMP (2 mg/kg i.p.) or sterile saline on days 7, 9 and 11 after ICH. Rats in EE also received training on beam (walking) and tray (skilled reaching) tasks 30 min after each injection. Walking (beam and ladder task), skilled reaching (tray and staircase tasks) and neurological deficits (NDS) were repeatedly assessed. We predicted that EE would improve recovery and that AMP would further enhance it. Results showed that EE, but not AMP, significantly and consistently improved recovery on the beam and ladder task. Neither treatment significantly affected skilled reaching. Lesion volume was not significantly different among groups (overall average: 44.6 mm(3) of tissue lost +/-15.3 S.D.). In conclusion, EE provides modest benefit for striatal ICH whereas AMP does not. This suggests that AMP will not provide substantial benefit to those patients with severe ICH affecting the basal ganglia.
右旋苯丙胺（AMP）与体育活动（如在横梁上行走）相结合，可改善啮齿动物皮质运动系统缺血性损伤后的恢复情况。我们测试了AMP是否能促进大鼠脑出血（ICH）后的恢复。通过立体定向向纹状体注射胶原酶产生中度大小的脑出血。五天后，将大鼠置于环境丰富笼子（EE）或对照条件下（标准笼子群居），直至脑出血后4周安乐死。在脑出血后第7、9和11天，给动物注射AMP（2mg/kg腹腔注射）或无菌生理盐水。EE组的大鼠在每次注射后30分钟还接受横梁（行走）和托盘（熟练够取）任务的训练。反复评估行走（横梁和梯子任务）、熟练够取（托盘和楼梯任务）和神经功能缺损（NDS）。我们预测EE会改善恢复情况，而AMP会进一步增强恢复。结果表明，EE而非AMP能显著且持续地改善横梁和梯子任务的恢复。两种治疗方法对熟练够取均无显著影响。各组间的损伤体积无显著差异（总体平均值：损失44.6立方毫米组织±15.3标准差）。总之，EE对纹状体脑出血有一定益处，而AMP则没有。这表明AMP对那些患有影响基底神经节的严重脑出血患者不会有实质性益处。
