Neuroscience and Mental Health Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
Department of Psychology, Faculty of Science, University of Alberta, Edmonton, AB, Canada.
Neurorehabil Neural Repair. 2022 Dec;36(12):788-799. doi: 10.1177/15459683221137342. Epub 2022 Nov 16.
The formation and degradation of an intracerebral hemorrhage causes protracted cell death, and an extended window for intervention. Experimental studies find that rehabilitation mitigates late cell death, with accelerated hematoma clearance as a potential mechanism.
We assessed whether early, intense, enriched rehabilitation (ER, environmental enrichment and massed skills training) enhances functional benefit, reduces brain injury, and augments hematoma clearance.
In experiment 1, rats (n = 56) were randomized to intervention in the light (-L) or dark phase (-D) of their housing cycle, then to 10 days of ER or control (CON) treatment after collagenase-induced striatal intracerebral hemorrhage (ICH). ER rats were treated from 5 to 14 days after ICH. Behavior and residual hematoma volume was assessed on day 14. In experiment 2, rats (n = 72) were randomized to ER-D10, ER-D20, or CON-D. ER rats completed 10 or 20 days of training in the dark. Rats were euthanized on day 60 for histology. In both experiments, behavioral assessment was completed pre-ICH, pre-ER (day 4 post-ICH), and post-ER (experiment 1: days 13-14; experiment 2: days 16-17 and 30-31).
Reaching intensity was high but similar between ER-D10 and ER-L10. Unlike previous work, rehabilitation did not alter skilled reaching or hematoma resolution. Varying ER duration also did not affect reaching success or lesion volume.
In contrast to others, and under these conditions, our findings show that striatal ICH was generally unresponsive to rehabilitation. This highlights the difficulty of replicating and extending published work, perhaps owing to small inter-study differences.
脑出血的形成和降解会导致细胞持续死亡，并为干预提供更长的窗口期。实验研究发现，康复治疗可以减轻晚期细胞死亡，血肿清除加速可能是其潜在机制。
我们评估了早期、强烈、丰富的康复治疗（ER，环境丰富和技能集中训练）是否能提高功能获益、减少脑损伤和增加血肿清除。
在实验 1 中，大鼠（n = 56）随机分为在其居住周期的光照（-L）或黑暗（-D）阶段接受干预，然后在胶原酶诱导的纹状体脑出血（ICH）后接受 10 天的 ER 或对照（CON）治疗。ER 大鼠从 ICH 后第 5 天到第 14 天接受治疗。在第 14 天评估行为和残余血肿体积。在实验 2 中，大鼠（n = 72）随机分为 ER-D10、ER-D20 或 CON-D。ER 大鼠在黑暗中完成 10 或 20 天的训练。大鼠在第 60 天被安乐死进行组织学检查。在这两个实验中，行为评估分别在 ICH 前、ER 前（ICH 后第 4 天）和 ER 后（实验 1：第 13-14 天；实验 2：第 16-17 天和 30-31 天）进行。
达到的强度很高，但 ER-D10 和 ER-L10 之间相似。与之前的工作不同，康复治疗并没有改变熟练的抓握或血肿溶解。ER 持续时间的变化也没有影响抓握成功率或病变体积。
与其他人的研究结果相反，在这些条件下，我们的发现表明纹状体 ICH 通常对康复治疗没有反应。这突出了复制和扩展已发表工作的难度，这可能是由于研究之间的微小差异。
