从生物化学到生理学：量热法的联系

From biochemistry to physiology: the calorimetry connection.

作者信息

Hansen Lee D, Russell Donald J, Choma Christin T

机构信息

Calorimetry Sciences Corp., 890 W 410 N, Suite A, Lindon, UT 84042, USA.

出版信息

Cell Biochem Biophys. 2007;49(2):125-40. doi: 10.1007/s12013-007-0049-y.

DOI:10.1007/s12013-007-0049-y

PMID:17906367

Abstract

This article provides guidelines for selecting optimal calorimetric instrumentation for applications in biochemistry and biophysics. Applications include determining thermodynamics of interactions in non-covalently bonded structures, and determining function through measurements of enzyme kinetics and metabolic rates. Specific examples illustrating current capabilities and methods in biological calorimetry are provided. Commercially available calorimeters are categorized by application and by instrument characteristics (isothermal or temperature-scanning, reaction vessel volume, heat rate detection limit, fixed or removable reaction vessels, etc.). Advantages and limitations of commercially available calorimeters are listed for each application in biochemistry, biophysics, and physiology.

摘要

本文提供了为生物化学和生物物理学应用选择最佳量热仪器的指南。应用包括确定非共价键结构中相互作用的热力学，以及通过测量酶动力学和代谢率来确定功能。文中给出了说明生物量热法当前能力和方法的具体示例。市售量热仪按应用和仪器特性（等温或温度扫描、反应容器体积、热速率检测限、固定或可移动反应容器等）进行分类。针对生物化学、生物物理学和生理学中的每种应用，列出了市售量热仪的优缺点。