Laboratory of Biothermodynamics and Drug Design / Institute of Biotechnology, Graičiūno 8, Vilnius, LT-02241, Lithuania.
Int J Mol Sci. 2009 Jun 18;10(6):2752-2762. doi: 10.3390/ijms10062752.
Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions.
目前文献中的等温滴定量热法（ITC）数据在测量蛋白质-配体结合反应的焓值方面存在相对较高的误差。滴定量热计需要通用的验证标准。已经提出了几种无机盐共沉淀和缓冲质子化反应作为可能的焓标准。使用这些建议的标准反应，对包括 VP-ITC、ITC200 和 Nano ITC-III 在内的几种商业量热仪的性能进行了验证。
