海洋脂质的生物合成研究。35. 使用放射性标记的类异戊二烯前体证明海绵中甾醇的从头生物合成。

Biosynthetic studies of marine lipids. 35. The demonstration of de novo sterol biosynthesis in sponges using radiolabeled isoprenoid precursors.

Silva C J, Wünsche L, Djerassi C

Department of Chemistry, Stanford University, CA 94305.

Comp Biochem Physiol B. 1991;99(4):763-73. doi: 10.1016/0305-0491(91)90140-9.

Abstract

De novo sterol biosynthesis in the sponges Tethya aurantia and Aplysina fistularis was investigated, using sodium [5,5-3H]-mevalonate, [1-3H]-farnesol and [3-3H]-squalene. [3-3H]-Squalene was found to be the best precursor for demonstrating de novo sterol biosynthesis in a wider range of sponges. 2. By feeding [3-3H]-squalene and using cell-free techniques, the de novo sterol biosynthesis was established in 18 sponges belonging to nine orders. Among these sponges were Axinella polypoides and Axinella verrucosa which had previously been thought to be incapable of de novo sterol biosynthesis based on work with radiolabeled lanosterol, cycloartenol, mevalonate, and acetate. 3. In contrast to earlier assumptions, it is likely that all sponges are capable of de novo sterol biosynthesis.

摘要

使用[5,5-³H]甲羟戊酸钠、[1-³H]法尼醇和[3-³H]角鲨烯，对橙黄海绵（Tethya aurantia）和管指海绵（Aplysina fistularis）中的从头甾醇生物合成进行了研究。发现[3-³H]角鲨烯是在更广泛的海绵中证明从头甾醇生物合成的最佳前体。2. 通过投喂[3-³H]角鲨烯并使用无细胞技术，在属于九个目的18种海绵中确立了从头甾醇生物合成。这些海绵包括多枝轴海绵（Axinella polypoides）和疣轴海绵（Axinella verrucosa），基于对放射性标记羊毛甾醇、环阿屯醇、甲羟戊酸和乙酸盐的研究，它们此前被认为无法进行从头甾醇生物合成。3. 与早期的假设相反，所有海绵都有可能进行从头甾醇生物合成。