Biosynthetic studies of marine lipids 36. The origin of common sterol side chains in eleven sponges using [3-3H]-squalene.

1. [3-3H]-Squalene was fed to 11 marine sponges containing a mixture of "common" sterol side chains. All of these sponges possess significant quantities of cholesterol, but their ability to biosynthesize it differs widely. 2. All the sponges possess significant quantities of delta 22 sterols, yet none of them was able to introduce the delta 22 double bond. 22-Dehydro-24-norcholesterol and 24-methyl-22-dehydro-27-norcholesterol side chains also originate from the diet. 3. These sponges biosynthesized between 40 and 80% of their sterols, a typical value being 70%. The remainder is derived from the diet or by modification of dietary sterols.