The lethal factor of the stonefish (Synanceja horrida) venom, designated as the stonustoxin, was purified to homogeneity by a two-step procedure on Sephacryl S-200 High Resolution (HR) gel permeation and DEAE Bio-Gel A anion exchange chromatography. 2. Stonustoxin has a native mol. wt of 148,000 and an isoelectric point of 6.9. 3. SDS-polyacrylamide gel electrophoresis revealed two subunits (designated alpha and beta) with mol. wts of 71,000 and 79,000, respectively. 4. The amino acid composition of both subunits and the N-terminal amino acid sequence of the beta subunit were also determined. 5. Purified stonustoxin had an LD50 of 0.017 microgram/g which is 22-fold more potent than that of the crude venom. 6. The toxin exhibited potent haemolytic activity in vitro and edema-inducing activity with a minimum edema dose (MED) of 0.15 micrograms in mouse paw. The edema effect was not antagonized by diphenhydramine.
摘要
石头鱼（玫瑰毒鲉）毒液的致死因子，命名为石鱼毒素，通过在Sephacryl S - 200高分辨率（HR）凝胶过滤和DEAE Bio - Gel A阴离子交换色谱上的两步法纯化至同质。2. 石鱼毒素的天然分子量为148,000，等电点为6.9。3. SDS - 聚丙烯酰胺凝胶电泳显示两个亚基（分别命名为α和β），分子量分别为71,000和79,000。4. 还测定了两个亚基的氨基酸组成和β亚基的N端氨基酸序列。5. 纯化的石鱼毒素的半数致死量为0.017微克/克，比粗毒液的效力高22倍。6. 该毒素在体外表现出强大的溶血活性和水肿诱导活性，在小鼠爪中的最小水肿剂量（MED）为0.15微克。水肿效应未被苯海拉明拮抗。
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