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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Sagi A, Homola E, Laufer H

Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.

Comp Biochem Physiol B. 1991;99(4):879-82. doi: 10.1016/0305-0491(91)90157-9.

The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558-52,652 DPM/hr per gland) between individual prawns. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. Hemolymph from males contained 17.3 +/- 9.5 ng/ml methyl farnesoate. 6. Female hemolymph contained 24 +/- 13.1 ng/ml methyl farnesoate.

鉴定了罗氏沼虾的下颌器官，并在[甲基-³H]-甲硫氨酸存在的情况下进行培养。2. 从雄性和雌性培养腺体及培养基中提取并定量放射性标记的法尼酸甲酯：观察到个体对虾之间合成速率存在差异（每个腺体每小时1558 - 52,652 每分钟衰变数）。3. 在培养的任何其他组织中均未观察到同位素显著掺入己烷可萃取物质中。4. 以顺反式（非生物异构体）为内标，使用正相高效液相色谱法测定罗氏沼虾血淋巴中法尼酸甲酯的浓度。5. 雄性血淋巴中法尼酸甲酯含量为17.3±9.5纳克/毫升。6. 雌性血淋巴中法尼酸甲酯含量为24±13.1纳克/毫升。

Sagi A, Homola E, Laufer H

Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.

Comp Biochem Physiol B. 1991;99(4):879-82. doi: 10.1016/0305-0491(91)90157-9.

The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558-52,652 DPM/hr per gland) between individual prawns. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. Hemolymph from males contained 17.3 +/- 9.5 ng/ml methyl farnesoate. 6. Female hemolymph contained 24 +/- 13.1 ng/ml methyl farnesoate.

鉴定了罗氏沼虾的下颌器官，并在[甲基-³H]-甲硫氨酸存在的情况下进行培养。2. 从雄性和雌性培养腺体及培养基中提取并定量放射性标记的法尼酸甲酯：观察到个体对虾之间合成速率存在差异（每个腺体每小时1558 - 52,652 每分钟衰变数）。3. 在培养的任何其他组织中均未观察到同位素显著掺入己烷可萃取物质中。4. 以顺反式（非生物异构体）为内标，使用正相高效液相色谱法测定罗氏沼虾血淋巴中法尼酸甲酯的浓度。5. 雄性血淋巴中法尼酸甲酯含量为17.3±9.5纳克/毫升。6. 雌性血淋巴中法尼酸甲酯含量为24±13.1纳克/毫升。