采用高效液相色谱法对血淋巴中甲法尼酯水平进行定量分析。

Quantification of methyl farnesoate levels in hemolymph by high-performance liquid chromatography.

作者信息

Borst D W, Tsukimura B

机构信息

Department of Biological Sciences, Illinois State University, Normal 61761.

出版信息

J Chromatogr. 1991 May 24;545(1):71-8. doi: 10.1016/s0021-9673(01)88696-9.

DOI:10.1016/s0021-9673(01)88696-9

PMID:1864901

Abstract

Methyl farnesoate (MF) is an acyclic sesquiterpenoid that has been detected in hemolymph and other tissues of crustaceans and insects. This paper describes a rapid and sensitive method for measuring MF in crustacean hemolymph. Extracts of hemolymph samples were separated by normal-phase high-performance liquid chromatography (5-microns silica, 250 x 4.6 mm I.D., 1.3% diethyl ether in hexane) and detected by UV (220 nm). The limit of detection with this method was less than 250 pg/ml. This method should be useful for studying the physiological functions of MF in crustaceans and other arthropods.

摘要

法尼酸甲酯（MF）是一种无环倍半萜类化合物，已在甲壳类动物和昆虫的血淋巴及其他组织中检测到。本文描述了一种快速灵敏的方法来测定甲壳类动物血淋巴中的MF。血淋巴样品提取物通过正相高效液相色谱法（5微米硅胶，内径250×4.6毫米，己烷中含1.3%乙醚）进行分离，并用紫外光（220纳米）进行检测。该方法的检测限低于250皮克/毫升。此方法对于研究MF在甲壳类动物和其他节肢动物中的生理功能应是有用的。