食蟹猴（猕猴）乳汁中α-乳白蛋白和β-乳球蛋白的鉴定。

Identification of alpha-lactalbumin and beta-lactoglobulin in cynomolgus monkey (Macaca fascicularis) milk.

Azuma N, Yamauchi K

Department of Agricultural Chemistry, University of Tokyo, Japan.

Comp Biochem Physiol B. 1991;99(4):917-21. doi: 10.1016/0305-0491(91)90164-9.

Abstract

An electrophoretic analysis of whey protein from cynomolgus monkey milk revealed that its constituents are more similar to bovine milk than human milk, i.e. cynomolgus monkey milk whey contains, besides alpha-lactalbumin-like protein (LaP), another predominant component similar to bovine beta-lactoglobulin (LgP), in its electrophoretic behavior on both disc- and SDS-polyacrylamide gel electrophoreses. 2. The amino acid composition of LaP shows close similarity to that of human alpha-lactalbumin, and LaP forms an immunoprecipitin line with anti-human alpha-lactalbumin rabbit antiserum. The homology between LaP and alpha-lactalbumin was further confirmed by an analysis of the N-terminal amino acid sequence. 3. LgP is not immunologically identical to bovine beta-lactoglobulin, but its amino acid composition is similar. The result of the N-terminal amino acid sequence analysis of LgP (up to the 26th residue) strongly suggests homology between this protein and beta-lactoglobulin.

摘要

对食蟹猴乳清蛋白进行的电泳分析表明，其成分与牛乳的成分比与人乳的成分更相似，即在圆盘聚丙烯酰胺凝胶电泳和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中，食蟹猴乳清除了含有α - 乳白蛋白样蛋白（LaP）外，还含有另一种与牛β - 乳球蛋白（LgP）相似的主要成分。2. LaP的氨基酸组成与人α - 乳白蛋白的氨基酸组成非常相似，并且LaP与抗人α - 乳白蛋白兔抗血清形成免疫沉淀线。通过对N端氨基酸序列的分析进一步证实了LaP与α - 乳白蛋白之间的同源性。3. LgP与牛β - 乳球蛋白在免疫学上并不完全相同，但其氨基酸组成相似。LgP的N端氨基酸序列分析结果（直至第26个残基）强烈表明该蛋白与β - 乳球蛋白之间存在同源性。