猫乳清蛋白：α-乳白蛋白、β-乳球蛋白和溶菌酶的鉴定、分离及初步表征

Feline whey proteins: identification, isolation and initial characterization of alpha-lactalbumin, beta-lactoglobulin and lysozyme.

Halliday J A, Bell K, McKenzie H A, Shaw D C

Department of Physiology and Pharmacology, University of Queensland, St Lucia, Brisbane, QLD.

Comp Biochem Physiol B. 1990;95(4):773-9. doi: 10.1016/0305-0491(90)90316-l.

Abstract

Both alpha-lactalbumin and beta-lactoglobulin-like proteins were detected in the whey fraction of feline milk by immunoblotting with rabbit antisera to alpha-lactalbumin and beta-lactoglobulin, respectively. 2. alpha-Lactalbumin was found to occur in both glycosylated and unglycosylated forms in approximately equal concentrations. No polymorphism of feline alpha-lactalbumin was found. 3. Feline beta-lactoglobulin-like proteins produced complex electrophoretic patterns that appear to be determined by three distinct loci. Between two and five genetic variants are expressed by each locus. 4. Lysozyme was detected at levels of approximately 1 mg/ml in skim milk. 5. The identifications of the proteins as alpha-lactalbumin, beta-lactoglobulin and lysozyme were confirmed by determination of N-terminal amino acid sequences.

摘要

通过分别用抗α-乳白蛋白和β-乳球蛋白的兔抗血清进行免疫印迹分析，在猫乳的乳清部分检测到了α-乳白蛋白和β-乳球蛋白样蛋白。2. 发现α-乳白蛋白以糖基化和非糖基化形式存在，且浓度大致相等。未发现猫α-乳白蛋白的多态性。3. 猫β-乳球蛋白样蛋白产生复杂的电泳图谱，似乎由三个不同的基因座决定。每个基因座表达两到五个遗传变体。4. 在脱脂乳中检测到溶菌酶的水平约为1毫克/毫升。5. 通过测定N端氨基酸序列，证实了这些蛋白质为α-乳白蛋白、β-乳球蛋白和溶菌酶。