Tao Ran, Hu Miao-Feng, Lou Jin-Tu, Lei Yong-Liang
Central Laboratory, Children's Hospital, School of Medicine, Zhejiang University, 57 Zhugan Lane, Hangzhou 310003, Zhejiang Province, China.
World J Gastroenterol. 2007 Nov 7;13(41):5497-500. doi: 10.3748/wjg.v13.i41.5497.
To explore the effects of H pylori infection on gap-junctional intercellular communication (GJIC) and proliferation of gastric epithelial cells in vitro.
A human gastric epithelial cell line (SGC-7901) cultured on coverslips was exposed overnight to intact H pylori (CagA(+) or CagA(-) strains) and sonicated extracts, respectively. GJIC between the cells was detected by fluorescence redistribution after photobleaching (FRAP) technique. Proliferation of SGC cells was determined by methylthiazolyl tetrazolium (MTT) assay.
When compared with control in which cells were cultured with simple medium alone, both CagA(+) and CagA(-) H pylori isolates could inhibit GJIC (CagA(+): F = 57.98, P < 0.01; CagA(-): F = 29.59, P < 0.01) and proliferation (CagA(+): F = 42.65, P < 0.01; CagA(-): F = 58.14, P < 0.01) of SGC-7901 cells. Compared with CagA(-) strains, CagA(+) H pylori more significantly down-regulated GJIC of gastric cells (intact H pylori: t = 13.86, P < 0.01; sonicated extracts: t = 11.87, P < 0.01) and inhibited proliferation gastric cells to a lesser extent in vitro (intact H pylori: t = 3.06, P < 0.05; sonicated extracts: t = 3.94, P < 0.01).
Compared with CagA(-) H pylori strains, CagA(+) strains down-regulate GJIC of gastric epithelial cells more significantly and inhibit proliferation of gastric cells to a lesser extent in vitro. H pylori, especially CagA(+) strains, may play an important role in gastric carcinogenesis.
探讨幽门螺杆菌感染对体外胃上皮细胞间隙连接细胞通讯（GJIC）及增殖的影响。
将培养在盖玻片上的人胃上皮细胞系（SGC-7901）分别用完整的幽门螺杆菌（细胞毒素相关基因A阳性或阴性菌株）和超声破碎提取物处理过夜。采用光漂白后荧光再分布（FRAP）技术检测细胞间的GJIC。通过甲基噻唑基四氮唑（MTT）法测定SGC细胞的增殖。
与仅用单纯培养基培养细胞的对照组相比，细胞毒素相关基因A阳性和阴性幽门螺杆菌分离株均可抑制SGC-7901细胞的GJIC（细胞毒素相关基因A阳性：F = 57.98，P < 0.01；细胞毒素相关基因A阴性：F = 29.59，P < 0.01）及增殖（细胞毒素相关基因A阳性：F = 42.65，P < 0.01；细胞毒素相关基因A阴性：F = 58.14，P < 0.01）。与细胞毒素相关基因A阴性菌株相比，细胞毒素相关基因A阳性幽门螺杆菌更显著地下调胃细胞的GJIC（完整幽门螺杆菌：t = 13.86，P < 0.01；超声破碎提取物：t = 11.87，P < 0.01），且在体外对胃细胞增殖的抑制作用较小（完整幽门螺杆菌：t = 3.06，P < 0.05；超声破碎提取物：t = 3.94，P < 0.01）。
与细胞毒素相关基因A阴性幽门螺杆菌菌株相比，细胞毒素相关基因A阳性菌株更显著地下调胃上皮细胞的GJIC，且在体外对胃细胞增殖的抑制作用较小。幽门螺杆菌，尤其是细胞毒素相关基因A阳性菌株，可能在胃癌发生中起重要作用。
