Metel'skiĭ S T
Biofizika. 2007 Jul-Aug;52(4):722-6.
It has been found that the thickness of the unstirred layer of liquid, determined by glycine, increases from 247 +/- 12 to 319 +/- 14 mkm (p < 0.001) as the concentration of glycine increases from 5 to 40 mM; in the case of determination by glucose, it increases from 316 +/- 13 to 380 +/- 26 mkm, as the concentration of glucose increases from 10 to 40 mM, due to the fact that nutrients at higher concentrations penetrate to a greater depth to the basis of villi. The occurrence of the positive weak correlation between the layer thickness, determined by glucose, maltose, saccharose, alanine, leucine, and glial in rats and the parameter characterizing the rate of absorption (the maximum response of the short-circuit current to nutrient) contradicts the assumption that the transporters are distributed uniformly along the height of villus, and nutrients do not arrive at transporters located between the villi at a greater depth. Probably, the transporters are unevenly distributed along the height of villi. The method proposed may serve as a tool for studying the topography of distribution of the corresponding transporters for nutrients throughout the villus surface.
已发现，由甘氨酸测定的未搅拌液层厚度，随着甘氨酸浓度从5 mM增加到40 mM，从247±12微米增加到319±14微米（p<0.001）；在由葡萄糖测定的情况下，随着葡萄糖浓度从10 mM增加到40 mM，它从316±13微米增加到380±26微米，这是因为较高浓度的营养物质渗透到绒毛基部更深的位置。在大鼠中，由葡萄糖、麦芽糖、蔗糖、丙氨酸、亮氨酸和胶质测定的层厚度与表征吸收速率的参数（短路电流对营养物质的最大反应）之间存在正弱相关，这与转运体沿绒毛高度均匀分布以及营养物质不会到达绒毛之间更深位置的转运体的假设相矛盾。可能，转运体沿绒毛高度分布不均匀。所提出的方法可作为研究整个绒毛表面营养物质相应转运体分布地形的工具。
