El-Ansary Afaf K, Ahmed Samia A, Aly Sanaa A
Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, Egypt.
Indian J Exp Biol. 2007 Sep;45(9):791-801.
With a view to clarify the induction of the "Crabtree consequence" in liver cells of S. mansoni infected mice, the curative effect of oil extract of C. longa was tested and compared to praziquantel (PZQ) the effective drug against all schistosome species occurring in man. Protein, glucose, glucose-6-phopsphatase, AMP-deaminase, adensoine deaminase, urea concentration, pyravate kinase (PK), phosphoenol pyruvate carboxykinase (PEPCK) and PK/PEPCK ratio were estimated. In addition, worm burden and ova count in mice infected with S. mansoni were elucidated. The result showed that C. longa normalized the concentration of protein, glucose, AMP-deaminase and adenosine deaminase, which were changed by infection. Moreover, it lowered pyruvate kinase level, while PZQ-treatment induced more elevation of this enzyme. PZQ was more effective in lowering worm burden while C. longa extract was more potent in reducing egg count.
为了阐明曼氏血吸虫感染小鼠肝细胞中“克氏效应”的诱导情况，测试了长柄胡椒油提取物的治疗效果，并与吡喹酮（PZQ）进行比较，吡喹酮是治疗人类所有血吸虫种类的有效药物。对蛋白质、葡萄糖、葡萄糖-6-磷酸酶、AMP脱氨酶、腺苷脱氨酶、尿素浓度、丙酮酸激酶（PK）、磷酸烯醇丙酮酸羧激酶（PEPCK）以及PK/PEPCK比值进行了评估。此外，还对感染曼氏血吸虫的小鼠的虫负荷和虫卵计数进行了研究。结果表明，长柄胡椒使因感染而改变的蛋白质、葡萄糖、AMP脱氨酶和腺苷脱氨酶的浓度恢复正常。此外，它降低了丙酮酸激酶水平，而吡喹酮治疗则使该酶水平进一步升高。吡喹酮在降低虫负荷方面更有效，而长柄胡椒提取物在减少虫卵计数方面更有效。
