曼氏血吸虫抗性、敏感和未感染的光滑双脐螺的排泄分泌蛋白的比较研究。
Comparative study of excretory-secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata.
机构信息
Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia.
QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia.
出版信息
Parasit Vectors. 2019 Sep 14;12(1):452. doi: 10.1186/s13071-019-3708-0.
BACKGROUND
Schistosomiasis is a harmful neglected tropical disease caused by infection with Schistosoma spp., such as Schistosoma mansoni. Schistosoma must transition within a molluscan host to survive. Chemical analyses of schistosome-molluscan interactions indicate that host identification involves chemosensation, including naïve host preference. Proteomic technique advances enable sophisticated comparative analyses between infected and naïve snail host proteins. This study aimed to compare resistant, susceptible and naïve Biomphalaria glabrata snail-conditioned water (SCW) to identify potential attractants and deterrents.
METHODS
Behavioural bioassays were performed on S. mansoni miracidia to compare the effects of susceptible, F1 resistant and naïve B. glabrata SCW. The F1 resistant and susceptible B. glabrata SCW excretory-secretory proteins (ESPs) were fractionated using SDS-PAGE, identified with LC-MS/MS and compared to naïve snail ESPs. Protein-protein interaction (PPI) analyses based on published studies (including experiments, co-expression, text-mining and gene fusion) identified S. mansoni and B. glabrata protein interaction. Data are available via ProteomeXchange with identifier PXD015129.
RESULTS
A total of 291, 410 and 597 ESPs were detected in the susceptible, F1 resistant and naïve SCW, respectively. Less overlap in ESPs was identified between susceptible and naïve snails than F1 resistant and naïve snails. F1 resistant B. glabrata ESPs were predominately associated with anti-pathogen activity and detoxification, such as leukocyte elastase and peroxiredoxin. Susceptible B. glabrata several proteins correlated with immunity and anti-inflammation, such as glutathione S-transferase and zinc metalloproteinase, and S. mansoni sporocyst presence. PPI analyses found that uncharacterised S. mansoni protein Smp_142140.1 potentially interacts with numerous B. glabrata proteins.
CONCLUSIONS
This study identified ESPs released by F1 resistant, susceptible and naïve B. glabrata to explain S. mansoni miracidia interplay. Susceptible B. glabrata ESPs shed light on potential S. mansoni miracidia deterrents. Further targeted research on specific ESPs identified in this study could help inhibit B. glabrata and S. mansoni interactions and stop human schistosomiasis.
背景
血吸虫病是一种由血吸虫属寄生虫感染引起的有害的被忽视的热带病，例如曼氏血吸虫。血吸虫必须在软体动物宿主内完成转换才能存活。血吸虫与软体动物相互作用的化学分析表明，宿主识别涉及化学感觉，包括幼稚宿主偏好。蛋白质组学技术的进步使得在感染和幼稚的蜗牛宿主蛋白之间进行复杂的比较分析成为可能。本研究旨在比较抗性、易感和幼稚的光滑双脐螺（Biomphalaria glabrata）条件水（SCW），以鉴定潜在的引诱物和驱避物。
方法
对曼氏血吸虫的尾蚴进行行为生物测定，以比较敏感、F1 抗性和幼稚的 B. glabrata SCW 的效果。使用 SDS-PAGE 对 F1 抗性和敏感的 B. glabrata SCW 分泌蛋白（ESPs）进行分离，通过 LC-MS/MS 进行鉴定，并与幼稚蜗牛的 ESP 进行比较。基于已发表的研究（包括实验、共表达、文本挖掘和基因融合）的蛋白质-蛋白质相互作用（PPI）分析确定了曼氏血吸虫和 B. glabrata 蛋白的相互作用。数据可通过 ProteomeXchange 获得，标识符为 PXD015129。
结果
在敏感、F1 抗性和幼稚的 SCW 中分别检测到 291、410 和 597 个 ESP。敏感蜗牛和幼稚蜗牛之间的 ESP 重叠较少，而 F1 抗性蜗牛和幼稚蜗牛之间的重叠较多。F1 抗性 B. glabrata 的 ESP 主要与抗病原体活性和解毒作用相关，如白细胞弹性蛋白酶和过氧化物酶。敏感的 B. glabrata 的几种蛋白质与免疫和抗炎作用相关，如谷胱甘肽 S-转移酶和锌金属蛋白酶，以及曼氏血吸虫的孢囊存在相关。PPI 分析发现，未鉴定的曼氏血吸虫蛋白 Smp_142140.1 可能与许多 B. glabrata 蛋白相互作用。
结论
本研究鉴定了 F1 抗性、敏感和幼稚的 B. glabrata 释放的 ESP，以解释曼氏血吸虫尾蚴的相互作用。敏感的 B. glabrata 的 ESP 揭示了潜在的曼氏血吸虫尾蚴驱避物。对本研究中鉴定的特定 ESP 进行进一步的靶向研究可能有助于抑制 B. glabrata 和曼氏血吸虫的相互作用，阻止人类血吸虫病的发生。
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