Kaur Pushpindar, Singh Rattan
Department of Zoology, Panjab University, Chandigarh 160 014, India.
Indian J Exp Biol. 2007 Sep;45(9):807-11.
Stavudine (Zerit, d4T) is widely used as an anti HIV infection drug. It prevents HIV by altering the genetic material of healthy cells but causes mutations in mitochondrial and nuclear DNA. It also produces clastogenic effects in mice. In the present investigation, comet assay test was applied to evaluate the possible genomic damage caused by stavudine and also the ameliorating effects of garlic oil and vitamin E against its genotoxicity in different organs of mice. Two different doses of garlic oil (low and high dose) and vitamin E were administered to mice separately and in combination for six consecutive days followed by a dose of stavudine. The mice were sacrificed after 24, 48 and 72 h of stavudine administration. Both the antioxidants (vitamin E and garlic oil) separately and in combination reduced the genotoxicity of stavudine. The protective effects of high doses of garlic oil were more pronounced as compared to vitamin E administered group.
司他夫定（齐多夫定，d4T）被广泛用作抗HIV感染药物。它通过改变健康细胞的遗传物质来预防HIV，但会导致线粒体和核DNA发生突变。它在小鼠中也产生致断裂效应。在本研究中，应用彗星试验来评估司他夫定可能造成的基因组损伤，以及大蒜油和维生素E对其在小鼠不同器官中的遗传毒性的改善作用。将两种不同剂量的大蒜油（低剂量和高剂量）和维生素E分别及联合给予小鼠，连续6天，随后给予一剂司他夫定。在给予司他夫定24、48和72小时后处死小鼠。抗氧化剂（维生素E和大蒜油）单独及联合使用均降低了司他夫定的遗传毒性。与给予维生素E的组相比，高剂量大蒜油的保护作用更明显。
