Li Wen-Gui, Wang Hong, Zhu You-Ming
Institute of Infectious and Parasitic Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Oct;23(10):911-3.
To investigate the changes of cytokines of splenocytes in mice immunized by mix recombinant BCG-EmII/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and challenged by Em protoscoleces.
BALB/c mice were vaccinated subcutaneously and intranasally by the vaccine respectively. In the eighth week of vaccination they were Challenged by Em protoscoleces.In the eighteenth week of infection they were killed for spleen. After splenocytes were separated,their culture was stimulated by EmAg or ConA and their supernatants were gathered. The levels of IL-2, IFN-gamma, TNF-alpha and IL-4 were measured by ELISA kit. Blank vector, BCG and PBS were served as control.
In the groups of vaccine immunization, the levels of IFN-gamma and TNF-alpha increased obviously but the level of IL-4 decreased. The level of TNF-alpha in subcutaneous group was higher than that in intranasal group.
Th1 response was induced in mice immunized by mix recombinant BCG-EmII/3 and BCG-Em14-3-3 vaccine of Em against the challenge by Em protoscoleces. Subcutaneous injection with this vaccine may be a good way of vaccination.
研究多房棘球绦虫（Em）混合重组卡介苗BCG-EmII/3和BCG-Em14-3-3疫苗免疫小鼠并经Em原头节攻击后脾细胞细胞因子的变化。
分别采用皮下和滴鼻方式用该疫苗免疫BALB/c小鼠。免疫第8周用Em原头节攻击小鼠。感染第18周处死小鼠取脾脏，分离脾细胞，用EmAg或ConA刺激培养后收集上清液，采用ELISA试剂盒检测IL-2、IFN-γ、TNF-α和IL-4水平。以空载体、卡介苗和PBS作为对照。
疫苗免疫组IFN-γ和TNF-α水平明显升高，IL-4水平降低。皮下组TNF-α水平高于滴鼻组。
Em混合重组卡介苗BCG-EmII/3和BCG-Em14-3-3疫苗免疫小鼠可诱导Th1反应以抵抗Em原头节攻击。皮下注射该疫苗可能是一种较好的免疫接种方式。
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