包含DNA复制起点的猿猴病毒40基因组片段的分离与增殖。

Isolation and propagation of a segment of the simian virus 40 genome containing the origin of DNA replication.

作者信息

Shenk T E, Berg P

出版信息

Proc Natl Acad Sci U S A. 1976 May;73(5):1513-7. doi: 10.1073/pnas.73.5.1513.

DOI:10.1073/pnas.73.5.1513

PMID:179089

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430327/

Abstract

Heteroduplex DNA molecules formed from two DNAs that differ from each other by a deletion can be cleaved at the mismatched region (a deletion loop) with the single-strand-specific S1 endonuclease. A heteroduplex DNA molecule, constructed from the DNA of simian virus 40 (SV40) mutant with a deletion of the map region 0.54-0.55 and the DNA of a second SV40 mutant having a deletion of the map segment 0.70-0.73, is cleaved twice with S1 endonuclease. One of the products is a DNA fragment of about 0.13 the length of SV40 DNA which contains the origin of SV40 DNA replication (0.67 on the SV40 DNA map).

摘要

由两条因缺失而彼此不同的DNA形成的异源双链DNA分子，可被单链特异性S1核酸内切酶在错配区域（缺失环）处切割。一个异源双链DNA分子，由猴病毒40（SV40）突变体的DNA构建而成，该突变体在图谱区域0.54 - 0.55处有缺失，以及第二个SV40突变体的DNA，该突变体在图谱片段0.70 - 0.73处有缺失，用S1核酸内切酶切割两次。其中一个产物是长度约为SV40 DNA 0.13的DNA片段，它包含SV40 DNA复制起点（在SV40 DNA图谱上为0.67）。