• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状和线性猴病毒40 DNA在重组方面存在差异。

Circular and linear simian virus 40 DNAs differ in recombination.

作者信息

Dorsett D, Deichaite I, Winocour E

出版信息

Mol Cell Biol. 1985 Apr;5(4):869-80. doi: 10.1128/mcb.5.4.869-880.1985.

DOI:10.1128/mcb.5.4.869-880.1985
PMID:2985972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366792/
Abstract

Linear forms of simian virus 40 (SV40) DNA, when added to transfection mixtures containing circular SV40 and phi X174 RFI DNAs, enhanced the frequency of SV40/phi X174 recombination, as measured by infectious center in situ plaque hybridization in monkey BSC-1 cells. The sequences required for the enhancement of recombination by linear DNA reside within the SV40 replication origin/regulatory region (nucleotides 5,171 to 5,243/0 to 128). Linearization of phi X174 RFI DNA did not increase the recombination frequency. The SV40/phi X174 recombinant structures arising from transfections supplemented with linear forms of origin-containing SV40 DNA contained phi X174 DNA sequences interspersed within tandem head-to-tail repeats derived from the recombination-enhancing linear DNA. Evidence is presented that the tandem repeats are not formed by homologous recombination and that linear forms of SV40 DNA must compete with circular SV40 DNA for the available T antigen to enhance recombination. We propose that the enhancement of recombination by linear SV40 DNA results from the entry of that DNA into a rolling circle type of replication pathway which generates highly recombinogenic intermediates.

摘要

将猴病毒40（SV40）DNA的线性形式添加到含有环状SV40和φX174 RFI DNA的转染混合物中时，如通过猴BSC - 1细胞中的感染中心原位噬菌斑杂交所测定的，会提高SV40/φX174重组的频率。线性DNA增强重组所需的序列位于SV40复制起点/调控区域内（核苷酸5171至5243/0至128）。φX174 RFI DNA的线性化并未增加重组频率。在补充了含起点的SV40 DNA线性形式的转染中产生的SV40/φX174重组结构，含有穿插在源自增强重组的线性DNA的串联头对头重复序列中的φX174 DNA序列。有证据表明串联重复不是由同源重组形成的，并且SV40 DNA的线性形式必须与环状SV40 DNA竞争可用的T抗原以增强重组。我们提出，线性SV40 DNA增强重组是由于该DNA进入滚环型复制途径，该途径产生高度重组的中间体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/7ac20c4b8134/molcellb00100-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/1bf8a857976a/molcellb00100-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/926860f0ebea/molcellb00100-0289-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/7ac20c4b8134/molcellb00100-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/1bf8a857976a/molcellb00100-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/926860f0ebea/molcellb00100-0289-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b126/366792/7ac20c4b8134/molcellb00100-0290-a.jpg

相似文献

1
Circular and linear simian virus 40 DNAs differ in recombination.环状和线性猴病毒40 DNA在重组方面存在差异。
Mol Cell Biol. 1985 Apr;5(4):869-80. doi: 10.1128/mcb.5.4.869-880.1985.
2
Quantitation of a simian virus 40 nonhomologous recombination pathway.猿猴病毒40非同源重组途径的定量分析。
J Virol. 1983 Oct;48(1):218-28. doi: 10.1128/JVI.48.1.218-228.1983.
3
Indiscriminate recombination in simian virus 40-infected monkey cells.猿猴病毒40感染的猴细胞中的随机重组。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4861-5. doi: 10.1073/pnas.77.8.4861.
4
Structure of simian virus 40-phiX174 recombinant genomes isolated from single cells.从单细胞中分离出的猿猴病毒40-φX174重组基因组的结构
J Virol. 1983 Oct;48(1):229-38. doi: 10.1128/JVI.48.1.229-238.1983.
5
The complete 30-base-pair origin region of bacteriophage phi X174 in a plasmid is both required and sufficient for in vivo rolling-circle DNA replication and packaging.质粒中噬菌体φX174完整的30个碱基对的起源区域对于体内滚环DNA复制和包装而言既是必需的也是充分的。
Eur J Biochem. 1985 Jun 18;149(3):579-84. doi: 10.1111/j.1432-1033.1985.tb08964.x.
6
Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.猴α-卫星重复序列在体外猿猴病毒40复制系统中的同源重组：重组与DNA复制的可能关联
Mol Cell Biol. 1994 Jun;14(6):4173-82. doi: 10.1128/mcb.14.6.4173-4182.1994.
7
Termination and reinitiation signals of bacteriophage phi X174 rolling circle DNA replication.噬菌体φX174滚环DNA复制的终止和重新起始信号
Virology. 1986 Oct 30;154(2):357-68. doi: 10.1016/0042-6822(86)90461-7.
8
Structure of simian virus 40-adeno-associated virus recombinant genomes.猴病毒40-腺相关病毒重组基因组的结构
J Virol. 1985 Nov;56(2):457-65. doi: 10.1128/JVI.56.2.457-465.1985.
9
Initiation and termination of the bacteriophage phi X174 rolling circle DNA replication in vivo: packaging of plasmid single-stranded DNA into bacteriophage phi X174 coats.噬菌体φX174滚环DNA复制在体内的起始与终止：质粒单链DNA包装进噬菌体φX174衣壳
Nucleic Acids Res. 1982 Nov 11;10(21):6849-63. doi: 10.1093/nar/10.21.6849.
10
Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication.含有噬菌体φX174 DNA复制起点序列的重组质粒DNA的构建与特性分析。
Nucleic Acids Res. 1981 Jul 24;9(14):3335-54. doi: 10.1093/nar/9.14.3335.

引用本文的文献

1
Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain.进行性多灶性白质脑病，即人类大脑中由 JC 病毒引起的脱髓鞘疾病的分子生物学、流行病学和发病机制。
Clin Microbiol Rev. 2012 Jul;25(3):471-506. doi: 10.1128/CMR.05031-11.
2
Does human bocavirus infection depend on helper viruses? A challenging case report.人博卡病毒感染是否依赖辅助病毒？一例具有挑战性的病例报告。
Virol J. 2011 Aug 29;8:417. doi: 10.1186/1743-422X-8-417.
3
Hairpin structures are the primary amplification products: a novel mechanism for generation of inverted repeats during gene amplification.

本文引用的文献

1
The SOS regulatory system of Escherichia coli.大肠杆菌的SOS调控系统。
Cell. 1982 May;29(1):11-22. doi: 10.1016/0092-8674(82)90085-x.
2
Recombination of transfected DNAs in vertebrate cells in culture.培养的脊椎动物细胞中转染DNA的重组。
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3476-80. doi: 10.1073/pnas.81.11.3476.
3
High mutation frequency in DNA transfected into mammalian cells.转染到哺乳动物细胞中的DNA具有高突变频率。
发夹结构是主要的扩增产物：基因扩增过程中产生反向重复序列的一种新机制。
Mol Cell Biol. 1994 Dec;14(12):7782-91. doi: 10.1128/mcb.14.12.7782-7791.1994.
4
Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.腺相关病毒位点特异性整合到附加体形成的重组连接。
J Virol. 1995 Nov;69(11):6917-24. doi: 10.1128/JVI.69.11.6917-6924.1995.
5
Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.用于哺乳动物细胞的分子间重组测定法，该方法可产生携带同源和非同源连接点的重组体。
Mol Cell Biol. 1987 Jun;7(6):2248-55. doi: 10.1128/mcb.7.6.2248-2255.1987.
6
Linear DNA must have free ends to transform rat cells efficiently.线性DNA必须具有游离末端才能有效地转化大鼠细胞。
Mol Gen Genet. 1987 Jan;206(1):121-5. doi: 10.1007/BF00326546.
7
Structure of simian virus 40-adeno-associated virus recombinant genomes.猴病毒40-腺相关病毒重组基因组的结构
J Virol. 1985 Nov;56(2):457-65. doi: 10.1128/JVI.56.2.457-465.1985.
8
Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication.线性猴病毒40 DNA片段呈现出滚环复制的倾向。
Mol Cell Biol. 1985 Jul;5(7):1787-90. doi: 10.1128/mcb.5.7.1787-1790.1985.
9
Functional comparison of transactivation by simian immunodeficiency virus from rhesus macaques and human immunodeficiency virus type 1.恒河猴猿猴免疫缺陷病毒与人类免疫缺陷病毒1型反式激活作用的功能比较
J Virol. 1988 Dec;62(12):4523-32. doi: 10.1128/JVI.62.12.4523-4532.1988.
10
Carcinogen-induced DNA amplification in vitro: overreplication of the simian virus 40 origin region in extracts from carcinogen-treated CO60 cells.致癌物诱导的体外DNA扩增：致癌剂处理的CO60细胞提取物中猿猴病毒40起源区域的过度复制。
Mol Cell Biol. 1990 Jan;10(1):75-83. doi: 10.1128/mcb.10.1.75-83.1990.
Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9. doi: 10.1073/pnas.80.10.3015.
4
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.DNA转入小鼠L细胞过程中的同源重组模型：DNA末端在重组过程中的作用。
Mol Cell Biol. 1984 Jun;4(6):1020-34. doi: 10.1128/mcb.4.6.1020-1034.1984.
5
Tumorigenic activity of polyoma virus and SV40 DNAs in newborn rodents.多瘤病毒和猿猴空泡病毒40 DNA在新生啮齿动物中的致瘤活性。
Virology. 1984 May;135(1):53-64. doi: 10.1016/0042-6822(84)90116-8.
6
Mutagenesis of a shuttle vector plasmid in mammalian cells.穿梭载体质粒在哺乳动物细胞中的诱变
Mol Cell Biol. 1984 Mar;4(3):435-41. doi: 10.1128/mcb.4.3.435-441.1984.
7
How damaged is the biologically active subpopulation of transfected DNA?转染DNA的生物活性亚群受损程度如何？
Mol Cell Biol. 1984 Mar;4(3):387-98. doi: 10.1128/mcb.4.3.387-398.1984.
8
DNA sequence studies of simian virus 40 chromosomal excision and integration in rat cells.猴病毒40在大鼠细胞中染色体切除与整合的DNA序列研究。
J Mol Biol. 1984 Mar 25;174(1):55-84. doi: 10.1016/0022-2836(84)90365-6.
9
Recombination between simian virus 40 and adeno-associated virus: virion coinfection compared to DNA cotransfection.猿猴病毒40与腺相关病毒之间的重组：病毒粒子共感染与DNA共转染的比较。
Virology. 1984 Apr 15;134(1):125-37. doi: 10.1016/0042-6822(84)90278-2.
10
Differences in intracellular DNA ligation after microinjection and transfection.显微注射和转染后细胞内DNA连接的差异。
Mol Cell Biol. 1984 Feb;4(2):240-6. doi: 10.1128/mcb.4.2.240-246.1984.