真核生物DNA在大肠杆菌中的复制与转录。
Replication and transcription of eukaryotic DNA in Escherichia coli.
作者信息
Morrow J F, Cohen S N, Chang A C, Boyer H W, Goodman H M, Helling R B
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7. doi: 10.1073/pnas.71.5.1743.
Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.
经EcoRI限制性内切酶切割产生的、编码18S和28S核糖体RNA的非洲爪蟾DNA扩增片段，已在体外与细菌质粒pSC101连接；重组分子已通过转化导入大肠杆菌。这些同时含有真核和原核DNA的重组质粒能在大肠杆菌中稳定复制。从携带这些质粒的大肠杆菌微小细胞中分离出的RNA能与扩增的非洲爪蟾rDNA杂交。
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