蛋白酶敏感型荧光纳米纤维。
Protease-sensitive fluorescent nanofibers.
作者信息
Law Benedict, Weissleder Ralph, Tung Ching-Hsuan
机构信息
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
出版信息
Bioconjug Chem. 2007 Nov-Dec;18(6):1701-4. doi: 10.1021/bc070054z. Epub 2007 Oct 5.
Abstract
We report the design and synthesis of enzyme-responsive nanofibers. The fibers are composed of self-assembled hydrophobic beta-sheet peptides incorporating protease-sensitive domains, fluorescent reporters, and hydrophilic poly(ethylene glycol) (PEG) units. Using urokinase plasminogen activator (uPA) as a model system, nanofibers were developed to release fluorescent fragments upon uPA incubation. These protease-sensitive nanofibers may have considerable biomedical applications as diagnostic sensors or for protease-assisted drug deliveries.
摘要
我们报告了酶响应性纳米纤维的设计与合成。这些纤维由自组装的疏水β-折叠肽组成，其中包含蛋白酶敏感结构域、荧光报告基团和亲水性聚乙二醇（PEG）单元。以尿激酶型纤溶酶原激活剂（uPA）作为模型系统，开发出了纳米纤维，使其在与uPA孵育时释放荧光片段。这些蛋白酶敏感的纳米纤维作为诊断传感器或用于蛋白酶辅助药物递送可能具有相当大的生物医学应用价值。
相似文献
1
Protease-sensitive fluorescent nanofibers.
Bioconjug Chem. 2007 Nov-Dec;18(6):1701-4. doi: 10.1021/bc070054z. Epub 2007 Oct 5.
2
Design and synthesis of a near-infrared fluorescent nanofiber precursor for detecting cell-secreted urokinase activity.
Anal Biochem. 2011 May 1;412(1):26-33. doi: 10.1016/j.ab.2011.01.010. Epub 2011 Jan 13.
3
Structural modification of protease inducible preprogrammed nanofiber precursor.
Biomacromolecules. 2008 Feb;9(2):421-5. doi: 10.1021/bm7012026. Epub 2008 Jan 5.
4
Design, synthesis, and characterization of urokinase plasminogen-activator-sensitive near-infrared reporter.
Chem Biol. 2004 Jan;11(1):99-106. doi: 10.1016/j.chembiol.2003.12.017.
5
Ligand interaction between urokinase-type plasminogen activator and its receptor probed with 8-anilino-1-naphthalenesulfonate. Evidence for a hydrophobic binding site exposed only on the intact receptor.
Biochemistry. 1994 Aug 2;33(30):8991-7. doi: 10.1021/bi00196a017.
6
alpha-2 Macroglobulin receptor/Ldl receptor-related protein(Lrp)-dependent internalization of the urokinase receptor.
J Cell Biol. 1995 Dec;131(6 Pt 1):1609-22. doi: 10.1083/jcb.131.6.1609.
7
A regulatory hydrophobic area in the flexible joint region of plasminogen activator inhibitor-1, defined with fluorescent activity-neutralizing ligands. Ligand-induced serpin polymerization.
J Biol Chem. 2001 Apr 20;276(16):13077-86. doi: 10.1074/jbc.M009024200. Epub 2001 Jan 25.
8
Urokinase-type plasminogen activator is a preferred substrate of the human epithelium serine protease tryptase epsilon/PRSS22.
Blood. 2005 May 15;105(10):3893-901. doi: 10.1182/blood-2003-10-3501. Epub 2005 Feb 8.
9
Single-chain urokinase-type plasminogen activator does not possess measurable intrinsic amidolytic or plasminogen activator activities.
Biochemistry. 1991 Jun 11;30(23):5797-805. doi: 10.1021/bi00237a024.
10
Internalization of the urokinase-plasminogen activator inhibitor type-1 complex is mediated by the urokinase receptor.
J Biol Chem. 1992 May 5;267(13):9129-33.
引用本文的文献
1
Peptide-Mediated Nanocarriers for Targeted Drug Delivery: Developments and Strategies.
Pharmaceutics. 2024 Feb 6;16(2):240. doi: 10.3390/pharmaceutics16020240.
2
A Urinary Drug-Disposing Approach as an Alternative to Intravesical Chemotherapy for Treating Nonmuscle Invasive Bladder Cancer.
Cancer Res. 2022 Apr 1;82(7):1409-1422. doi: 10.1158/0008-5472.CAN-21-2897.
3
A combined approach of convection-enhanced delivery of peptide nanofiber reservoir to prolong local DM1 retention for diffuse intrinsic pontine glioma treatment.
Neuro Oncol. 2020 Oct 14;22(10):1495-1504. doi: 10.1093/neuonc/noaa101.
4
Recent development of highly sensitive protease assay methods: Signal amplification through enzyme cascades.
Biotechnol Bioprocess Eng. 2012;17(6):1113-1119. doi: 10.1007/s12257-012-0545-9. Epub 2013 Jan 4.
5
Enabling sublingual peptide immunization with molecular self-assemblies.
Biomaterials. 2020 May;241:119903. doi: 10.1016/j.biomaterials.2020.119903. Epub 2020 Feb 24.
6
Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties.
Adv Funct Mater. 2018 Oct 31;28(44). doi: 10.1002/adfm.201803969. Epub 2018 Sep 14.
7
Protease-Sensitive Nanomaterials for Cancer Therapeutics and Imaging.
Ind Eng Chem Res. 2017 May 24;56(20):5761-5777. doi: 10.1021/acs.iecr.7b00990. Epub 2017 Apr 24.
8
Smart Nanotransformers with Unique Enzyme-Inducible Structural Changes and Drug Release Properties.
Biomacromolecules. 2016 Jun 13;17(6):2040-9. doi: 10.1021/acs.biomac.6b00227. Epub 2016 May 24.
9
Well-defined, reversible boronate crosslinked nanocarriers for targeted drug delivery in response to acidic pH values and cis-diols.
Angew Chem Int Ed Engl. 2012 Mar 19;51(12):2864-9. doi: 10.1002/anie.201107144. Epub 2012 Jan 17.
10
NEAR-INFRARED DYES: Probe Development and Applications in Optical Molecular Imaging.
Curr Org Synth. 2011 Aug;8(4):521-534. doi: 10.2174/157017911796117223.
本文引用的文献
1
Heparin binding nanostructures to promote growth of blood vessels.
Nano Lett. 2006 Sep;6(9):2086-90. doi: 10.1021/nl0613555.
2
Slow release of molecules in self-assembling peptide nanofiber scaffold.
J Control Release. 2006 Sep 28;115(1):18-25. doi: 10.1016/j.jconrel.2006.06.031. Epub 2006 Jul 8.
3
Polymer conjugates as anticancer nanomedicines.
Nat Rev Cancer. 2006 Sep;6(9):688-701. doi: 10.1038/nrc1958. Epub 2006 Aug 10.
4
Acceleration of amyloid beta-peptide aggregation by physiological concentrations of calcium.
J Biol Chem. 2006 Sep 22;281(38):27916-23. doi: 10.1074/jbc.M602061200. Epub 2006 Jul 26.
5
Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction.
Proc Natl Acad Sci U S A. 2006 May 23;103(21):8155-60. doi: 10.1073/pnas.0602877103. Epub 2006 May 12.
6
Delivering cancer stem cell therapies - a role for nanomedicines?
Eur J Cancer. 2006 Jun;42(9):1309-15. doi: 10.1016/j.ejca.2006.01.044. Epub 2006 May 8.
7
Peptide-based biomaterials for protease-enhanced drug delivery.
Biomacromolecules. 2006 Apr;7(4):1261-5. doi: 10.1021/bm050920f.
8
Peptides as novel smart materials.
Curr Opin Struct Biol. 2005 Aug;15(4):453-63. doi: 10.1016/j.sbi.2005.07.005.
9
Self-assembled peptide amphiphile nanofibers conjugated to MRI contrast agents.
Nano Lett. 2005 Jan;5(1):1-4. doi: 10.1021/nl0484898.
10
Optical zymography for specific detection of urokinase plasminogen activator activity in biological samples.
Anal Biochem. 2005 Mar 1;338(1):151-8. doi: 10.1016/j.ab.2004.11.039.
Zotero 插件