成人人类牙髓组织中表达ABCG2的侧群细胞。
Side population cells expressing ABCG2 in human adult dental pulp tissue.
作者信息
Honda M J, Nakashima F, Satomura K, Shinohara Y, Tsuchiya S, Watanabe N, Ueda M
机构信息
Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
出版信息
Int Endod J. 2007 Dec;40(12):949-58. doi: 10.1111/j.1365-2591.2007.01301.x. Epub 2007 Oct 4.
AIM
To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.
METHODOLOGY
Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.
RESULTS
SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.
CONCLUSION
Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
目的
通过Hoechst排除法研究成人牙髓组织中侧群（SP）细胞的存在情况。
方法
从第三磨牙获取成人牙髓来源的细胞。细胞用Hoechst 33342染色，然后分选成SP细胞或非SP细胞[主要群体（MP）细胞]。对这两种细胞类型进行细胞生长和逆转录聚合酶链反应（RT-PCR）分析比较。
结果
发现表达ABCG2、巢蛋白、Notch-1和α-平滑肌肌动蛋白的SP细胞频率在0.67%至1.02%之间。在维拉帕米存在的情况下，这种SP细胞特征消失。当在成骨培养基中培养时，这些SP细胞表达牙本质涎磷蛋白和牙本质基质蛋白-1。
结论
成人牙髓组织含有可分化为成牙本质细胞样细胞的SP细胞。
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