Szepesi Áron, Matula Zsolt, Szigeti Anna, Várady György, Szabó Gyula, Uher Ferenc, Sarkadi Balázs, Német Katalin
1 Research Centre for Natural Sciences, Hungarian Academy of Sciences , Budapest, Hungary .
Stem Cells Dev. 2015 Jan 15;24(2):244-52. doi: 10.1089/scd.2014.0177.
Periodontal ligament stem cells (PDLSCs) provide an important source for tissue regeneration and may become especially useful in the formation of osteogenic seeds. PDLSCs can be cultured, expanded, and differentiated in vitro; thus, they may be applied in the long-term treatment of the defects in the dental regions. Here we studied numerous potential markers allowing the selection of human PDLSCs with a maximum differentiation potential. We followed the expression of the ATP-binding cassette subfamily G member 2 (ABCG2) membrane transporter protein and isolated ABCG2-expressing cells by using a monoclonal antibody, recognizing the transporter at the cell surface in intact cells. The expression of the ABCG2 protein, corresponding to the so-called side-population phenotype in various tissue-derived stem cells, was found to be a useful marker for the selection of PDLSCs with enhanced osteogenic, chondrogenic, and adipogenic differentiation. These findings may have important applications in achieving efficient dental tissue regeneration by using stem cells from extracted teeth.
牙周膜干细胞（PDLSCs）为组织再生提供了重要来源，在成骨种子形成方面可能特别有用。PDLSCs可在体外培养、扩增和分化；因此，它们可应用于牙科区域缺损的长期治疗。在此，我们研究了众多潜在标志物，以筛选出具有最大分化潜能的人PDLSCs。我们追踪了ATP结合盒亚家族G成员2（ABCG2）膜转运蛋白的表达，并使用单克隆抗体分离出表达ABCG2的细胞，该抗体可识别完整细胞表面的转运蛋白。发现ABCG2蛋白的表达与各种组织来源干细胞中所谓的侧群表型相对应，是筛选具有增强成骨、软骨生成和脂肪生成分化能力的PDLSCs的有用标志物。这些发现可能在利用拔牙来源的干细胞实现高效牙组织再生方面具有重要应用。
