来自犊牛的非典型肠致病性大肠杆菌和非O157产志贺毒素大肠杆菌的eae和stx基因变体
Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves.
作者信息
Wani S A, Hussain I, Nabi A, Fayaz I, Nishikawa Y
机构信息
Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, SK University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar, India.
出版信息
Lett Appl Microbiol. 2007 Dec;45(6):610-5. doi: 10.1111/j.1472-765X.2007.02235.x. Epub 2007 Oct 4.
AIMS
To determine the subtypes of stx and eae genes of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and to ascertain the typical and atypical nature of EPEC.
METHODS AND RESULTS
One hundred and eighty-seven faecal samples from 134 diarrhoeic and 53 healthy calves were investigated for the presence of stx, eae and ehxA virulence genes by polymerase chain reaction and enzyme-linked immunosorbent assay. Subtype analysis of stx(1) exhibited stx(1c) in 13 (31.70%) isolates, while that of stx(2) revealed stx(2c) in eight (24.24%) and stx(2d) in two (6.06%) isolates. Subtyping of eae gene showed the presence of eae-beta, eae-eta and eae-zeta in two, three and four isolates respectively. None of the E. coli isolates possessed stx(2e), stx(2f), eae-alpha, eae-delta, eae-epsilon and eae-xi. All EPEC isolates were atypical.
CONCLUSIONS
stx(1), stx(1c), stx(2), stx(2c), stx(2d), eae-beta, eae-eta and eae-zeta subtypes are prevalent in STEC and EPEC isolates in India.
SIGNIFICANCE AND IMPACT OF THE STUDY
This is the first subtype analysis of stx(2) and eae genes of animal E. coli isolates in India and emphasizes the need to investigate their transmission to humans.
目的
确定来自犊牛的产志贺毒素大肠杆菌（STEC）和肠致病性大肠杆菌（EPEC）的stx和eae基因亚型，并确定EPEC的典型和非典型性质。
方法与结果
通过聚合酶链反应和酶联免疫吸附测定法，对134头腹泻犊牛和53头健康犊牛的187份粪便样本进行stx、eae和ehxA毒力基因检测。stx(1)亚型分析显示，13株（31.70%）分离株中有stx(1c)，而stx(2)亚型分析显示，8株（24.24%）分离株中有stx(2c)，2株（6.06%）分离株中有stx(2d)。eae基因亚型分析显示，分别有2株、3株和4株分离株存在eae-β、eae-η和eae-ζ。所有大肠杆菌分离株均不具有stx(2e)、stx(2f)、eae-α、eae-δ、eae-ε和eae-ξ。所有EPEC分离株均为非典型株。
结论
stx(1)、stx(1c)、stx(2)、stx(2c)、stx(2d)、eae-β、eae-η和eae-ζ亚型在印度的STEC和EPEC分离株中普遍存在。
研究的意义和影响
这是印度首次对动物大肠杆菌分离株的stx(2)和eae基因进行亚型分析，并强调了调查它们向人类传播的必要性。
Zotero 插件