Oh Jae Young, Do Kyung-Hyo, Jeong Jae Hong, Kwak SuMin, Choe Sujin, An Dongheui, Chae Jong-Chan, Lee Kwangjun, Seo Kwang-Won
Division of Biotechnology, Jeonbuk National University, Iksan 54596, Korea.
Advanced Institute of Environment and Bioscience, Jeonbuk National University, Iksan 54596, Korea.
J Vet Sci. 2025 Jan;26(1):e1. doi: 10.4142/jvs.24225. Epub 2024 Dec 2.
This study is essential for comprehending the zoonotic transmission, antimicrobial resistance, and genetic diversity of enteropathogenic (EPEC).
To improve our understanding of EPEC, this study focused on analyzing and comparing the genomic characteristics of EPEC isolates from humans and companion animals in Korea.
The whole genome of 26 EPEC isolates from patients with diarrhea and 20 EPEC isolates from companion animals in Korea were sequenced using the Illumina HiSeq X (Illumina, USA) and Oxford Nanopore MinION (Oxford Nanopore Technologies, UK) platforms.
Most isolates were atypical EPEC, and did not harbor the gene. The most prevalent virulence genes were found to be (humans: 61.5%; companion animals: 60.0%) followed by (humans: 46.2%; companion animals: 60.0%). Although pan-genome analyses showed no apparent correlation among the origin of the strains, virulence profiles, and antimicrobial resistance profiles, isolates included in clade A obtained from both humans and companion animals exhibited high similarity. Additionally, all the isolates included in clade A encoded the gene and did not encode the gene. The two isolates from companion animals harbored an incomplete bundle-forming pilus region encoding and . Moreover, the type IV secretion system-associated genes and were found in the -encoding isolates from humans.
Whole-genome sequencing enabled a more accurate analysis of the phylogenetic structure of EPEC and provided better insights into the understanding of EPEC epidemiology and pathogenicity.
本研究对于理解肠道致病性大肠杆菌（EPEC）的人畜共患病传播、抗菌药物耐药性及遗传多样性至关重要。
为增进对EPEC的了解，本研究着重分析和比较韩国人类及伴侣动物来源的EPEC分离株的基因组特征。
使用Illumina HiSeq X（美国Illumina公司）和Oxford Nanopore MinION（英国Oxford Nanopore Technologies公司）平台，对韩国腹泻患者的26株EPEC分离株及伴侣动物的20株EPEC分离株进行全基因组测序。
多数分离株为非典型EPEC，未携带[具体基因]基因。最常见的毒力基因是[具体基因1]（人类：61.5%；伴侣动物：60.0%），其次是[具体基因2]（人类：46.2%；伴侣动物：60.0%）。尽管泛基因组分析显示菌株来源、毒力谱和抗菌药物耐药谱之间无明显相关性，但从人类和伴侣动物获得的A分支中的分离株表现出高度相似性。此外，A分支中的所有分离株均编码[具体基因3]基因且不编码[具体基因4]基因。来自伴侣动物的两株分离株含有编码[具体基因5]和[具体基因6]的不完整束状菌毛区域。此外，在人类来源的编码[具体基因7]的分离株中发现了与IV型分泌系统相关的基因[具体基因8]和[具体基因9]。
全基因组测序能够更准确地分析EPEC的系统发育结构，并为理解EPEC的流行病学和致病性提供更好的见解。
