Kalnina Inta, Zvagule Tija, Gabruseva Natalija, Kirilova Jelena, Kurjane Natalja, Bruvere Ruta, Kesters Andris, Kizane Gunta, Kirilovs Georgijs, Meirovics Imants
Daugavpils University, Daugavpils, Latvia.
J Fluoresc. 2007 Nov;17(6):633-8. doi: 10.1007/s10895-007-0256-3. Epub 2007 Oct 9.
ABM (3-aminobenzanthrrone derivative) developed at the Riga Technical University, Riga, Latvia) has been previously shown as a potential probe for determination of the immune state of patients with different pathologies . The fist study (using probe ABM) of peripheral blood mononuclear cells (PBMC) membranes of 97 Chernobyl clean-up workers from Latvia was conducted in 1997. Now we repeatedly examine the same (n = 54) individuals in dynamics. ABM spectral parameters in PBMC suspension, fluorescence anisotropy and blood plasma albumin characteristics were recorded. In 1997 screening showed 5 different patterns of fluorescence spectra, from which in 2007 we obtained only two. These patterns of spectra had never been previously seen in healthy individuals or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc., examined by us. Patterns of ABM fluorescence spectra are associated with membrane anisotropy and conformational changes of blood plasma albumin. We observed that in dynamics 1997-2007 the lipid compartment of the membrane became more fluid while the lipid-protein interface became more rigid. The use of probe ANS and albumin auto-fluorescence allowed show conformational alterations in Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters significantly differ in observed groups of patients. These findings reinforce our understanding that that the cell membrane is a significant biological target of radiation. The role of the membrane in the expression and course of cell damage after radiation exposure must be considered. So ten years dynamic of PBMC membrane characteristics by ABM (spectral shift and anisotropy indexes) in Chernobyl clean-up workers reveal progressive trend toward certain resemblance with those of chronic B-cell lymphoid leukemia.
ABM（3-氨基苯并蒽酮衍生物，由拉脱维亚里加的里加技术大学研发）先前已被证明是一种用于测定不同病理患者免疫状态的潜在探针。1997年，对来自拉脱维亚的97名切尔诺贝利清理工人的外周血单个核细胞（PBMC）膜进行了首次研究（使用探针ABM）。现在，我们对相同的54名个体进行了动态重复检查。记录了PBMC悬浮液中的ABM光谱参数、荧光各向异性和血浆白蛋白特征。1997年的筛查显示有5种不同的荧光光谱模式，而到2007年我们只得到了两种。这些光谱模式在我们之前检查过的健康个体或患有结核病、多发性硬化症、类风湿性关节炎等疾病的患者中从未见过。ABM荧光光谱模式与膜各向异性和血浆白蛋白的构象变化有关。我们观察到，在1997年至2007年的动态过程中，膜的脂质部分变得更具流动性，而脂质-蛋白质界面变得更僵硬。使用探针ANS和白蛋白自发荧光能够显示切尔诺贝利清理工人血浆中的构象改变。需要注意的是，所有研究参数在观察到的患者组中都有显著差异。这些发现强化了我们的认识，即细胞膜是辐射的一个重要生物学靶点。必须考虑膜在辐射暴露后细胞损伤的表达和过程中的作用。因此，通过ABM（光谱位移和各向异性指数）对切尔诺贝利清理工人PBMC膜特征进行的十年动态研究揭示了其与慢性B细胞淋巴细胞白血病的某些相似性的渐进趋势。
