Daugavpils University, Daugavpils, Latvia.
J Fluoresc. 2011 May;21(3):923-7. doi: 10.1007/s10895-010-0608-2. Epub 2010 Feb 24.
The use of hydrophobic fluorescent probe ABM (benzanthrone derivative) and albumin autofluorescence allowed show conformational alterations in Chernobyl clean-up workers blood plasma. Results obtained in 1996-1997 suggest that acidic expansion of plasma albumin takes place. Latest data (2006-2008) result in splitting of albumin alterations onto two stages - acidic expansion and N-F transition. The N-F transition is accompanied by the blue shift of fluorescence spectra and dehydration of tryptophanyl region of albumin molecule. In 2007 obtained.patterns of ABM spectra had never been previously seen in examined healthy individuals or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc. Patterns of ABM fluorescence spectra are associated with conformational changes of blood plasma albumin. The use of probe ABM and albumin auto-fluorescence allowed show conformational alterations in albumin of Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters significantly differ in observed groups of patients. These findings reinforce our understanding that the blood plasma albumin is a significant biological target of radiation. It may be concluded that fluorescence characteristics are representative of radiation induced albumin alterations and its carrier function.
使用疏水荧光探针 ABM（苯并蒽酮衍生物）和白蛋白自体荧光，可以显示切尔诺贝利清理工人血浆中的构象变化。1996-1997 年的研究结果表明，血浆白蛋白发生酸性扩张。最新数据（2006-2008 年）表明，白蛋白的变化可分为两个阶段——酸性扩张和 N-F 转变。N-F 转变伴随着荧光光谱的蓝移和白蛋白分子色氨酸区域的去水合作用。2007 年获得的 ABM 光谱模式在之前检查的健康个体或结核病、多发性硬化症、类风湿性关节炎等患者中从未见过。ABM 荧光光谱模式与血浆白蛋白的构象变化有关。使用探针 ABM 和白蛋白自体荧光可以显示切尔诺贝利清理工人血浆白蛋白的构象变化。需要注意的是，在观察到的患者组中，所有研究的参数都有显著差异。这些发现增强了我们对血液白蛋白是辐射的重要生物靶标的理解。可以得出结论，荧光特性是辐射诱导白蛋白变化及其载体功能的代表性特征。
