ABCG2在人恶性胶质瘤中的表达及意义

[Expression and significance of ABCG2 in human malignant glioma].

作者信息

Chu Liang, Huang Qiang, Zhai De-Zhong, Zhu Qing, Huo Hong-Mei, Dong Jun, Qian Zhi-Yuan, Wang Ai-Dong, Lan Qing, Gao Yi-Lu

机构信息

Department of Neurosurgery/Brain Tumor Research Laboratory, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215004, P. R. China.

出版信息

Ai Zheng. 2007 Oct;26(10):1090-4.

PMID:17927879

Abstract

BACKGROUND & OBJECTIVE: ATP-binding cassette transporter protein ABCG2 is a marker derived from hematopoietic stem cells. However, its role in tumorigenesis and malignant progression of glioma is unclear. This study was to investigate the expression and significance of ABCG2 in gliomas of different malignant grades.

METHODS

A microarray chip containing glioma tissues of different malignant grades, implanted glioma xenografts in nude mice, spheroids of glioma cell lines and glioma stem cells was prepared and examined for the expression of ABCG2 with immunohistochemical staining.

RESULTS

The positive rate of ABCG2 was 26.8% in the 71 specimens of human glioma tissues, with 11.1% in grade I gliomas, 8% in grade II gliomas, 43.5% in grade III gliomas, and 42.9% in grade IV gliomas; it was significantly higher in grade III-IV gliomas than in grade I-II gliomas (chi2=10.710, P=0.001). The positive rate of ABCG2 was 100% in implanted glioma xenografts in nude mice, gliomas stem cells, and neural stem cells. It was also expressed in some normal tissues. The positive cells surrounded and invaded into vessels in glioma tissues.

CONCLUSIONS

ABCG2 is overexpressed in glioma stem cells, glioma tissues of higher grades, and implanted glioma xenografts. The positive cells distribute around vessels in glioma tissues.

摘要

背景与目的

ATP结合盒转运蛋白ABCG2是一种源自造血干细胞的标志物。然而，其在胶质瘤发生及恶性进展中的作用尚不清楚。本研究旨在探讨ABCG2在不同恶性程度胶质瘤中的表达及意义。

方法

制备包含不同恶性程度胶质瘤组织、裸鼠移植胶质瘤异种移植物、胶质瘤细胞系球体及胶质瘤干细胞的微阵列芯片，采用免疫组化染色检测ABCG2的表达。

结果

71例人胶质瘤组织标本中ABCG2阳性率为26.8%，其中Ⅰ级胶质瘤为11.1%，Ⅱ级胶质瘤为8%，Ⅲ级胶质瘤为43.5%，Ⅳ级胶质瘤为42.9%；Ⅲ-Ⅳ级胶质瘤的ABCG2阳性率显著高于Ⅰ-Ⅱ级胶质瘤（χ2=10.710，P=0.001）。裸鼠移植胶质瘤异种移植物、胶质瘤干细胞及神经干细胞中ABCG2阳性率均为100%。其在一些正常组织中也有表达。阳性细胞在胶质瘤组织中围绕并侵入血管。