Suzuki Ryo, Furuno Tadahide, Okamoto Keisuke, Teshima Reiko, Nakanishi Mamoru
Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
J Neuroimmunol. 2007 Dec;192(1-2):49-56. doi: 10.1016/j.jneuroim.2007.09.013. Epub 2007 Oct 24.
Previously, we showed that nerve-mast cell cross-talk can occur bidirectionally and that substance P is a mediator to activate mast cells. Here, we have studied the mediators to activate nerves cocultured with mast cells. Addition of antigen to the cocultures of superior cervical ganglia (SCG) and rat basophilic leukemia cells (RBLs) elicited Ca(2+) response in RBLs and after a lag period induced Ca(2+) signal in SCG neurites. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (purinergic receptor antagonist) or apyrase (ATP-hydrolyzing enzyme) reduced the Ca(2+) signals in neurites, indicating that ATP released from activated mast cells was one of important mediators to activate nerves.
此前，我们发现神经-肥大细胞之间的相互作用是双向的，且P物质是激活肥大细胞的一种介质。在此，我们研究了激活与肥大细胞共培养的神经的介质。将抗原添加到颈上神经节（SCG）和大鼠嗜碱性白血病细胞（RBLs）的共培养物中，可引起RBLs中的Ca(2+)反应，并在一段延迟期后诱导SCG神经突中的Ca(2+)信号。磷酸吡哆醛-6-偶氮苯-2',4'-二磺酸（嘌呤能受体拮抗剂）或腺苷三磷酸双磷酸酶（ATP水解酶）可降低神经突中的Ca(2+)信号，表明活化肥大细胞释放的ATP是激活神经的重要介质之一。
