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磷酸化簇在冠状病毒传染性支气管炎病毒核衣壳蛋白生物学中的作用

Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein.

作者信息

Spencer Kelly-Anne, Dee Michael, Britton Paul, Hiscox Julian A

机构信息

Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

出版信息

Virology. 2008 Jan 20;370(2):373-81. doi: 10.1016/j.virol.2007.08.016. Epub 2007 Nov 1.

DOI:10.1016/j.virol.2007.08.016
PMID:17931676
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7103301/
Abstract

The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster was involved in the recognition of viral RNA from non-viral RNA. The IBV N protein has been found to be essential for the successful recovery of IBV using reverse genetics systems. Rescue experiments indicated that phosphorylated N protein recovered infectious IBV more efficiently when compared to modified N proteins either partially or non-phosphorylated. Our data indicate that the phosphorylated form of the IBV N protein plays a role in virus biology.

摘要

冠状病毒传染性支气管炎病毒（IBV）核衣壳（N）蛋白是一种RNA结合蛋白，在两个保守簇处被磷酸化。RNA结合的动力学分析表明，C端磷酸化簇参与了病毒RNA与非病毒RNA的识别。已发现IBV N蛋白对于使用反向遗传学系统成功拯救IBV至关重要。拯救实验表明，与部分磷酸化或未磷酸化的修饰N蛋白相比，磷酸化的N蛋白能更有效地拯救出传染性IBV。我们的数据表明，IBV N蛋白的磷酸化形式在病毒生物学中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/c89a7be02fb5/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/dd0b15be6889/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/f1103658b19b/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/28aa85eeca1a/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/81fb1d482160/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/c89a7be02fb5/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/dd0b15be6889/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/f1103658b19b/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/28aa85eeca1a/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/81fb1d482160/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d44b/7103301/c89a7be02fb5/gr5_lrg.jpg

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