Cooney M K
Am J Med Technol. 1976 May;42(5):158-65.
A survey of cell culture systems for virus isolation is presented. Because there is not single cell culture system which will support the many viruses that may be encountered in clinical specimens, the characteristics and susceptibility of primary, diploid, and continuous cell cultures are reviewed. The primary cell culture system should be susceptible to infection with myxoviruses, paramyxoviruses, adenoviruses, and Coxsackie B viruses, since these are not consistently recognized in diploid cell cultures. Conversely, rhinoviruses, cytomegalovirus and varicella-zoster virus may be isolated only in diploid cell cultures. Upon consideration of expected viruses, and cell cultures available for isolation of these, one can choose a combination of a primary and a diploid cell system for virus isolation which will assure detection of most human virus groups known. The usefulness of continuous cell culture systems is generally limited to a very few specific viruses.
本文介绍了用于病毒分离的细胞培养系统调查。由于没有一种单一的细胞培养系统能够支持临床标本中可能遇到的多种病毒，因此对原代细胞、二倍体细胞和连续细胞培养物的特性及易感性进行了综述。原代细胞培养系统应该对黏液病毒、副黏液病毒、腺病毒和柯萨奇B病毒敏感，因为这些病毒在二倍体细胞培养物中并非总能被识别。相反，鼻病毒、巨细胞病毒和水痘-带状疱疹病毒可能只能在二倍体细胞培养物中分离出来。考虑到预期的病毒以及可用于分离这些病毒的细胞培养物，人们可以选择原代细胞系统和二倍体细胞系统的组合用于病毒分离，这将确保检测出已知的大多数人类病毒组。连续细胞培养系统的用途通常仅限于极少数特定病毒。
