Ciccarelli Francesca D, Doerks Tobias, von Mering Christian, Creevey Christopher J, Snel Berend, Bork Peer
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany.
Science. 2006 Mar 3;311(5765):1283-7. doi: 10.1126/science.1123061.
We have developed an automatable procedure for reconstructing the tree of life with branch lengths comparable across all three domains. The tree has its basis in a concatenation of 31 orthologs occurring in 191 species with sequenced genomes. It revealed interdomain discrepancies in taxonomic classification. Systematic detection and subsequent exclusion of products of horizontal gene transfer increased phylogenetic resolution, allowing us to confirm accepted relationships and resolve disputed and preliminary classifications. For example, we place the phylum Acidobacteria as a sister group of delta-Proteobacteria, support a Gram-positive origin of Bacteria, and suggest a thermophilic last universal common ancestor.
我们已经开发出一种可自动化的程序，用于重建生命之树，其分支长度在所有三个域中都具有可比性。这棵树基于191个具有测序基因组的物种中出现的31个直系同源基因的串联。它揭示了分类学分类中的域间差异。系统地检测并随后排除水平基因转移的产物提高了系统发育分辨率，使我们能够确认已被接受的关系，并解决有争议的和初步的分类。例如，我们将酸杆菌门置于δ-变形菌的姐妹群中，支持细菌的革兰氏阳性起源，并提出一个嗜热的最后普遍共同祖先。
