大肠杆菌中用于异丙醇生产的工程合成途径。
Engineered synthetic pathway for isopropanol production in Escherichia coli.
作者信息
Hanai T, Atsumi S, Liao J C
机构信息
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, CA 90095, USA.
出版信息
Appl Environ Microbiol. 2007 Dec;73(24):7814-8. doi: 10.1128/AEM.01140-07. Epub 2007 Oct 12.
Abstract
A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutylicum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers.
摘要
通过表达来自丙酮丁醇梭菌ATCC 824、大肠杆菌K-12 MG1655、拜氏梭菌NRRL B593和布氏嗜热厌氧菌HTD4的各种基因组合，在大肠杆菌中构建了一条合成途径来生产异丙醇。含有丙酮丁醇梭菌thl（乙酰辅酶A [CoA] 乙酰转移酶）、大肠杆菌atoAD（乙酰乙酰辅酶A转移酶）、丙酮丁醇梭菌adc（乙酰乙酸脱羧酶）和拜氏梭菌adh（仲醇脱氢酶）组合的菌株产量最高。该菌株在摇瓶中产生了81.6 mM异丙醇，在生产阶段的产率为43.5%（摩尔/摩尔）。据我们所知，这项工作是首次在大肠杆菌中生产异丙醇，其产量超过了天然生产者。
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